소개

HiPure FFPE RNA Kit supplies a simple and rapid RNA extraction for Formalin-fixed, 파라핀이 내장된 (FFPE) tissue and sections samples. This kit is based on silica gel column purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 30 분 (not including digestion time). RNA can be directly used for downstream applications such as RT-PCR, Northern blot, vitro translation and other experiments.

세부

명세서

원칙

This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 분. After lysis, samples are incubated at 80ºC for 15 분. Transfer to an adsorption column and RNA is adsorbed on the membrane, 단백질은 흡착되지 않고 여과로 제거됩니다. 단백질 및 기타 불순물을 세척한 후, RNA wasfinally eluted with low-salt buffer.

장점

• Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• 빠른 – 여러 샘플을 추출할 수 있습니다. 60 minutes by column method
• Safe – no phenol chloroform extraction required
• Sensitive – RNA can be recovered at the level of PG

키트 내용물

보관 및 안정성

Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. 하지만, 단기 보관 (DNase I up to 1 주, Proteinase K up to 8 주) 실온에서(15-25°C) 성능에 영향을 미치지 않습니다. The remaining kit components can be stored at roomtemperature (15-25°C) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안.

실험 데이터