소개

The HiPure Liquid RNA & miRNA Kit integrates phenol/구아니딘-based sample lysis and silica-membrane purification of total RNA. MagZol LS Reagent, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate designed to facilitate lysis of liquid sample and inhibit RNase. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable the use of up to 0.25ml liquid sample per mini 스핀 컬럼.

세부

명세서

원칙

0.25ml liquid samples are homogenized in 0.75ml MagZol LS Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (까지 100 µg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30–100µl of RNase-free water.

장점

• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Fast – isolation of several samples can be completed in 40 minutes by using the column purification method
• Safety – no phenol-chloroform extraction is required
• Sensitive – direct lysis of blood, 혈장, and other samples without separation of leukocytes

키트 내용물

보관 및 안정성

MagZol LS Reagent should be stored at 2-8°C upon arrival. 하지만, 단기 보관 (까지 24 주) 실온에서 (15-25℃) 성능에 영향을 미치지 않습니다. 나머지 키트 구성품은 실온에서 건조한 상태로 보관할 수 있습니다. (15-25℃) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안.