소개

Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 분. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

세부

명세서

원칙

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of a high concentration of ionizing agent (~와 같은 guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. 마지막으로, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

장점

• 빠른 – 여러 샘플을 추출할 수 있습니다. 30 분
• 고순도 – the purified RNA can be directly used in various downstream applications
• 높은 회복력 – RNA can be recovered at the level of PG
• 좋은 반복성 – silica gel column purification technology can obtain ideal results every time
• Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
• Sufficient components – the kit contains carried wall-breaking enzymes and glass beads

키트 내용물

보관 및 안정성

Buffer PHC should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. 하지만, 단기 보관 (DNase I up to 1 주, Buffer PHC up to 12 주) 실온에서(15-25°C) 성능에 영향을 미치지 않습니다. The remaining kit components can be stored at roomtemperature (15-25°C) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안.