This product is suitable for extracting total RNA from 50-150mg conventional plantor fungal samples as well as samples rich in polyphenolic and polysaccharides. This kit is based on silica gel column purification technology, and the extraction process does not require the use of toxic phenol chloroform extraction. The entire extraction process only takes 20-30 분. This kit adopts DNA filtration technology, which can efficiently filter and remove DNA. The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+ purification, nucleic acid protection, and in vitro translation.
세부
키트 내용물
| 내용물 | R415002 | D415003 |
| 정화 시간 | 50 준비 | 250 준비 |
| HiPure RNA Mini Columns | 50 | 250 |
| gDNA Filter Columns | 50 | 250 |
| 2ml 수집 튜브 | 100 | 500 |
| TCEP (1중) | 0.29 g | 5 엑스 0.29 g |
| Buffer EP | 1.0 밀리리터 | 5.0 밀리리터 |
| Buffer PSL | 50 밀리리터 | 250 밀리리터 |
| Buffer RW1 | 50 밀리리터 | 250 밀리리터 |
| Buffer RW2* | 20 밀리리터 | 2 엑스 50 밀리리터 |
| RNase Free Water | 10 밀리리터 | 30 밀리리터 |
보관 및 안정성
Except TCEP, the kit components can be stored dry at room temperature (15-25°C) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안. After receiving the product, it is recommended to store TCEP (dry powder) at -20-8°C. At low temperatures, Buffer PSL may form precipitates, dissolve it completely by 55°C water bath.
상품평
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