소개

The HiPure Minipreps system provides a fast, simple, and cost-effective 플라스미드 DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

세부

명세서

원칙

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. 단백질 및 기타 불순물을 세척한 후, 최종적으로 저염 완충액으로 핵산을 용출시켰습니다. (10mm 트리스, pH9.0, 0.5mm EDTA).

장점

• 고순도 – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, 등.
• 빠른 – silica gel column purification is much faster than ion exchange
• Ultra low endotoxin – content of endotoxin is less than 0.05 EU/μg, which can be directly used for cell transfection and animal injection, 등.

키트 내용물

보관 및 안정성

The kit components can be stored dry at room temperature (15-25℃) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.

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