소개

The HiPure 플라스미드 DNA Mega Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. 까지 10 mg high copy number plasmid DNA can be purified from 500 mL overnight culture.

세부

명세서

원칙

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. 단백질 및 기타 불순물을 세척한 후, 최종적으로 저염 완충액으로 핵산을 용출시켰습니다. (10mm 트리스, pH9.0, 0.5mm EDTA).

장점

• 고순도 – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, 등.
• 빠른 – silica gel column purification is much faster than ion exchange
• 높은 수율 – up to 10mg plasmid can be binded in one column
• Low endotoxin – the obtainedplasmid can be directly used for cell transfection and animal injection, 등.

키트 내용물

보관 및 안정성

The kit components can be stored dry at room temperature (15-25℃) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안. If any precipitates form in the buffers, warm at 37℃ to dissolve.

구매안내