소개

• HiPure 플라스미드 DNA 을 더한 96 전부:
  • Enables up to 96 mini-preps to be performed simultaneously.
  • Process completed in less than 45 minutes using the Vacuum Manifold (Qiavac 96).
  • Provides a fast, simple, and cost-effective method for high-throughput plasmid DNA extraction in routine molecular biology laboratory applications.
• 주요 특징들:
  • Utilizes silica-membrane technology, eliminating cumbersome steps associated with loose resins or slurries.
  • Plasmid DNA purified with the kit is immediately ready for use.
  • Eliminates the need for phenol extraction and ethanol precipitation.
  • High-quality plasmid DNA is eluted in a small volume of Tris buffer or water.

세부

명세서

원칙

• HiPure Plasmid Procedure:
  • Based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt.
  • Utilizes a unique silica membrane that replaces glass or silica slurries for plasmid DNA minipreps.
• Procedure Steps:
  1. Preparation and Clearing of Bacterial Lysate:
     • The alkaline method is used to prepare and clear bacterial lysate.
  2. DNA Binding:
     • Supernatant transferred to a column to bind DNA to the silica membrane.
  3. 세척 및 용출:
     • Proteins and other impurities washed away.
     • 최종적으로 저염 완충액으로 핵산을 용출시켰습니다. (10mm 트리스, pH9.0, 0.5mm EDTA).

장점

• 고순도 – purified plasmid can be directly used in sequencing, enzyme digestion PCR, 등.
• 빠른 – it takes only 60 minutes to complete the isolation
• 높은 수율 – up to 1mg plasmid can be bound in one column
• 경제 – high cost-performance

키트 내용물

보관 및 안정성

The kit components can be stored dry at room temperature (15-25℃) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안. If any precipitates form in the buffers, warm at 37℃ to dissolve. After the addition of RNase A， Buffer P1 is stable for 6 months when stored at 2-8°C.

구매안내