HiPure Soil RNA Kit

소개

HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.

세부

명세서

원칙

The hipure silica gel column is based on a glass fiber filter membrane with a high binding force. Under the condition of a high concentration of ionizing agent (~와 같은 guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonds and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. 마지막으로, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

장점

• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• 빠른 – extraction of several samples can be completed in 60 minutes by the column purification method
• Safe – no phenol-chloroform extraction required
• Sensitive – RNA can be purified at the level of PG

키트 내용물

보관 및 안정성

Buffer PHC should be stored at 2–8°C upon arrival. 하지만, 단기 보관 (까지 12 주) 실온에서 (15-25°C) 성능에 영향을 미치지 않습니다. 나머지 키트 구성품은 실온에서 건조한 상태로 보관할 수 있습니다. (15-25°C) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안. 전체 키트는 2~8°C에서 보관 가능, 하지만 이 경우에는, 버퍼는 사용하기 전에 다시 용해되어야 합니다.. 사용 시 모든 버퍼가 실온에 있는지 확인하십시오..

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