- Isolation of High-Quality Genomic DNA: The product enables rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples.
- Sample Processing Capacity: Capable of processing up to 100mg of stool samples and 500 mg of soil samples within 60 분.
- Innovative System Design: Combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of 스핀 컬럼 기술.
- Elimination of PCR Inhibitors: Effectively eliminates PCR-inhibiting compounds such as humic acid from soil samples during the purification process.
- Suitability for Various Applications: The purified DNA is suitable for PCR, restriction digestion, 및 차세대 시퀀싱.
- Reduction in Organic Extractions: No organic extractions are required, thus reducing plastic waste and hands-on time.
- Increased Efficiency: Allows for the processing of multiple samples in parallel, enhancing overall workflow efficiency.
명세서
| 특징 | 명세서 |
| 주요 기능 | Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 그릇 |
| 응용 | PCR, 서던 블롯 및 효소 소화, 등. |
| 정제방법 | 96 우물 접시 |
| 정제기술 | 실리카 기술 |
| 가공방법 | 수동 (원심분리 또는 진공) |
| 샘플 유형 | Soil, stool, other environmental samples |
| 샘플량 | Soil: 200-500mg Stool: 50-100mg Other environmental samples: 100-500mg |
| 용출량 | |
| 실행당 시간 | |
| 컬럼당 액체 운반량 | |
| 컬럼의 결합 수율 |
원칙
- Sample Homogenization and Lysis: Soil or stool samples are homogenized and treated in a specially formulated buffer containing detergent. This process effectively lyses bacteria, 누룩, and fungal samples.
- Removal of Contaminants: Our proprietary Absorber Solution is utilized to remove humic acid, proteins, polysaccharides, and other contaminants from the lysed sample.
- DNA Binding and Column Application: After adjusting binding conditions, the treated sample is applied to a DNA Mini Column, facilitating the binding of DNA to the column matrix.
- Rapid Wash Steps: Two rapid wash steps are employed to remove trace contaminants, ensuring the purity of the extracted DNA.
- Elution of Purified DNA: Pure DNA is eluted from the column in a low ionic strength buffer, providing high-quality, purified DNA ready for downstream applications.
- Direct Use in Downstream Applications: The purified DNA can be directly used in downstream applications without the need for further purification steps, streamlining laboratory workflows and saving time.
장점
- 빠른 – 여러 샘플을 추출할 수 있습니다. 40 분 (소화 후)
- 고순도 – the purified DNA can be directly used in various downstream applications
- 좋은 반복성 – 실리카 기술은 매번 이상적인 결과를 얻을 수 있습니다
- 높은 회복력 – PG 수준에서 DNA 복구 가능
키트 내용물
| 내용물 | D314401 | D314402 | D314403 |
| 정화 시간 | 1 엑스 96 준비 | 4 엑스 96 준비 | 20 엑스 96 준비 |
| HiPure DNA Plate | 1 | 4 | 20 |
| 96 Well Plate (2.2밀리리터) | 1 | 4 | 20 |
| 1.6ml Collection Plate | 1 | 4 | 20 |
| 0.8ml Collection Plate | 1 | 4 | 20 |
| 2ml Bead Tubes | 100 | 400 | 2000 |
| Buffer SOL | 100 밀리리터 | 360 밀리리터 | 2 엑스 900 밀리리터 |
| 완충기 SDS | 10 밀리리터 | 36 밀리리터 | 180 밀리리터 |
| 시약 DX | 1 밀리리터 | 1.8 밀리리터 | 9 밀리리터 |
| Buffer PS | 20 밀리리터 | 80 밀리리터 | 400 밀리리터 |
| Absorber Solution | 20 밀리리터 | 80 밀리리터 | 400 밀리리터 |
| Buffer GDP | 150 밀리리터 | 500 밀리리터 | 3 엑스 900 밀리리터 |
| 버퍼 GW2* | 50 밀리리터 | 100 밀리리터 | 4 엑스 200 밀리리터 |
| 버퍼 AE | 30 밀리리터 | 120 밀리리터 | 500 밀리리터 |
보관 및 안정성
- Absorber Solution Storage: Upon arrival, the Absorber Solution should be stored at 2-8°C. 하지만, it can also be stored at room temperature (15-25℃) for short-term periods (까지 24 주) without affecting its performance.
- Remaining Kit Components: The remaining components of the kit can be stored dry at room temperature (15-25℃). They remain stable for at least 18 이런 조건에서 몇 달 동안, ensuring their efficacy and performance over an extended period.
상품평
아직 상품평이 없습니다.