HiPure 대변 DNA 키트

Stool Sample in Molecular Biology Research:

1. Utility and Applications:
   • Stool samples are increasingly utilized in animal molecular genetics, population ecology, behavioral ecology, and intestinal disease diagnosis.
   • They contain gut microbial DNA, food residue sample DNA, and alimentary tract exfoliated cell DNA.
2. Challenges:
   • Low content of exfoliated cells in the digestive tract and degradation of genetic material pose primary challenges.
   • Presence of inhibitors such as polysaccharides, bile acids, bile salts, bile pigments, digestive juices, and mucus can affect PCR-based molecular analysis.

Importance of DNA Extraction:

Critical Role:

• • Selecting appropriate extraction methods is crucial for obtaining high-quality DNA and ensuring successful downstream detection of stool DNA.

HiPure 대변 DNA 키트:

1. Solution Provided:
   • The HiPure Stool DNA Kit from Magen Company offers a promising solution.
   • Features good universality, high cost-effectiveness, high yield, and purification.
2. Unique Features:
   • Utilizes a unique solution system and inhibitory factor adsorbent to efficiently remove various impurities in stool samples.
   • The purified DNA can be directly used for PCR, 정량적 PCR, 및 기타 응용 프로그램.
3. Efficiency and Ease of Use:
   • Enables rapid and reliable isolation of high-quality genomic DNA from various stool samples.
   • Capable of processing up to 100 mg of stool samples within 60 분.
   • Combines reversible nucleic acid binding properties with 스핀 컬럼 기술, eliminating PCR inhibiting compounds.
   • Suitable for PCR, restriction digestion, and next-generation sequencing applications.
4. Sustainability and Scalability:
   • Eliminates the need for organic extractions, reducing plastic waste and hands-on time.
   • Allows multiple samples to be processed in parallel, enhancing efficiency.

세부

명세서

원칙

Stool DNA Extraction Process:

1. Homogenization and Lysis:
   • Stool sample is homogenized and treated in a specially formulated buffer containing detergent.
   • This step effectively lyses bacteria, 누룩, and fungal samples present in the stool.
2. 오염물질 제거:
   • Our proprietary Absorber Solution is used to remove humic acid, proteins, polysaccharides, and other contaminants from the lysed sample.
3. Binding and Washing:
   • Adjusted binding conditions facilitate the attachment of DNA to a DNA Mini Column.
   • Two rapid wash steps are performed to remove trace contaminants, ensuring purity of the DNA.
4. Elution of Purified DNA:
   • Pure DNA is eluted from the column in a low ionic strength buffer.
   • The resulting purified DNA is ready for direct use in downstream applications without the need for further purification.

장점

• 고순도 – unique adsorbent can completely remove inhibitory factors
• 고농도 – maximum extraction of total DNA from stool samples
• 높은 회복력 – PG 수준에서 DNA 복구 가능
• 좋은 반복성 – 실리카 기술은 매번 이상적인 결과를 얻을 수 있습니다

키트 내용물

보관 및 안정성

Storage Instructions:

1. Proteinase K and Buffer PCI:
   • Upon arrival, 2~8°C에서 보관.
   • 단기 보관 (까지 12 주) 실온에서 (15-25℃) 성능에 영향을 미치지 않습니다.
2. Remaining Kit Components:
   • Store at room temperature (15-25℃).
   • Stable for at least 18 이런 조건에서 몇 달 동안.
3. Entire Kit:
   • Can be stored at 2–8°C.
   • Buffers should be redissolved before use if stored at this temperature.
   • Ensure all buffers are at room temperature when used.