하이퓨어 티슈&Blood DNA 맥시 컬럼 키트

• Challenges in DNA Extraction from Blood Samples:
  1. High Infectious Risk: Blood samples pose a significant infectious hazard to operators and the environment.
  2. Complex DNA Source: Blood DNA contains various types such as mitochondrial, 게놈의, and circulating DNA, leading to potential loss during extraction.
  3. Presence of Impurities: Blood samples contain impurities and inhibitory factors that can interfere with DNA extraction and downstream applications.
• Existing Extraction Methods:
  • Phenol-chloroform extraction, salting out, 등.
  • These methods typically involve pre-treatment steps to remove red blood cells and isolate white blood cells, which may result in loss of nucleic acid information.
• Product Benefits:
  • Fast and Easy Purification: Provides a rapid and straightforward method for total DNA 정제.
  • Suitability: Purifies total DNA (게놈의, 바이러스의, 미토콘드리아) from whole blood, 조직, 및 배양된 세포.
  • 애플리케이션: Ensures reliable DNA for PCR and Southern blotting assays.

명세서

원칙

1. Cell Breakdown: Your sample is lysed (broken open) and digested with enzymes to release DNA into solution.
2. Capture: The mixture is passed through a silica column. DNA sticks to the silica membrane, while proteins and other impurities don’t.
3. Washing Away Impurities: The column is washed to remove any remaining proteins and other unwanted molecules.
4. DNA Release: Pure DNA is eluted (washed off) the silica membrane with a special buffer solution. This purified DNA is then ready for further analysis.

장점

• 고품질 DNA – 다양한 다운스트림 애플리케이션을 충족합니다., PCR을 포함하여, qPCR, 효소 소화, 이종 교잡, 등.
• 빠른 – 백혈구 분리 없이, 유기 추출, 또는 에탄올 침전
• 단순한 – 모든 핵산은 직접 소화로 얻을 수 있다
• Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
• 폭넓은 적용성 – 다양한 액체 샘플 처리
• 경제 – excellent cost-effectiveness performance

키트 내용물

보관 및 안정성

Storage Recommendations:

• Proteinase K and RNase A: Upon arrival, 2~8°C에서 보관. 단기 보관 (까지 12 주) 실온에서 (15-25℃) is acceptable without affecting performance.
• Remaining Kit Components: Can be stored at room temperature (15-25℃) and remain stable for at least 18 이런 조건에서 몇 달 동안.

Purchase Guide