The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, 조직, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, 노던 블로팅, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
세부
명세서
| 특징 | 명세서 |
| 주요 기능 | Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column |
| 응용 | RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing |
| 정제방법 | 미니 스핀 컬럼 |
| 정제기술 | 실리카 기술 |
| 가공방법 | 수동 (원심분리 또는 진공) |
| 샘플 유형 | Animal soft tissue, 배양된 세포, lymphocytes, simple plant tissue |
| 샘플량 | 세포: ≤1 x 107 Animal tissue: 1-20 mg Plant leaves: 50-150 mg Yeast cells: 5 엑스 106 |
| Yield | 2-100μg |
| 용출량 | ≥50μl |
| 실행당 시간 | ≤15 minutes（1-24 samples） |
| 컬럼당 액체 운반량 | 800μl |
| 컬럼의 결합 수율 | 100µg |
원칙
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
장점
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- 빠른 – 여러 샘플을 추출할 수 있습니다. 20 minutes by column method
- Safe – no phenol chloroform extraction required
- Sensitive – RNA can be recovered at the level of PG
키트 내용물
| 내용물 | R401102 | R401103 |
| 정화 시간 | 50 준비 | 250 준비 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml 수집 튜브 | 50 | 250 |
| RTL Lysis Buffer | 50 밀리리터 | 200 밀리리터 |
| RNA Binding Buffer | 15 밀리리터 | 75 밀리리터 |
| Buffer RW1 | 50 밀리리터 | 200 밀리리터 |
| Buffer RW2* | 20 밀리리터 | 2 엑스 50 밀리리터 |
| RNase Free Water | 10 밀리리터 | 30 밀리리터 |
보관 및 안정성
The Kit can be stored dry at room temperature (15-25℃) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안.
상품평
아직 상품평이 없습니다.