This kit provides fast purification of high-quality RNA from cells, 조직, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, 노던 블로팅, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
세부
명세서
| 특징 | 명세서 |
| 주요 기능 | Isolation total RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 엑스 106 cell using two columns (gDNA removed column) |
| 응용 | RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing |
| 정제방법 | 미니 스핀 컬럼 |
| 정제기술 | 실리카 기술, DNA filtration technology |
| 가공방법 | 수동 (원심분리 또는 진공) |
| 샘플 유형 | Animal soft tissue, 배양된 세포, lymphocytes, simple plant tissue |
| 샘플량 | Cells:≤1 x 107 Animal tissue sample: 1-20 mg Plant tissue: 50-150 mg |
| Yield | 2-100μg |
| 용출량 | ≥50μl |
| 실행당 시간 | ≤25 minutes（1-24 samples） |
| 컬럼당 액체 운반량 | 800μl |
| 컬럼의 결합 수율 | 100µg |
원칙
The Kit isolates total RNA from up to 107 cells or 20 mg 조직. A short workflow enables RNA isolation with genomic DNA removal in less than 25 분. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.
장점
- Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- 빠른 – 여러 샘플을 추출할 수 있습니다. 25 minutes by column method
- Safe – no phenol chloroform extraction required
- Sensitive – RNA can be recovered at the level of PG
키트 내용물
| 내용물 | R411102 | D411103 |
| 정화 시간 | 50 준비 | 250 준비 |
| HiPure DNA Mini Columns | 50 | 250 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml 수집 튜브 | 100 | 2 엑스 250 |
| Buffer RLC | 50 밀리리터 | 200 밀리리터 |
| Buffer RW1 | 50 밀리리터 | 200 밀리리터 |
| Buffer RW2* | 12 밀리리터 | 2 엑스 50 밀리리터 |
| RNase Free Water | 10 밀리리터 | 30 밀리리터 |
보관 및 안정성
HiPureKit can be stored dry at room temperature (15-25℃) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.
상품평
아직 상품평이 없습니다.