소개
The Kit integrates phenol/guanidine-based lysis and silica-membrane purification of total RNA. MagZol 시약, included in the kits, is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate the lysis of samples and inhibit RNases. The high lysis efficiency of the reagent and the subsequent removal of contaminants by organic phase extraction enable extracting up to 200μg total purified RNA from less than 100mg of animal, plant, and fungal samples, 박테리아, 세포, and other samples.
세부
명세서
| 특징 | 명세서 |
| 주요 기능 | Isolation of total RNA (including miRNA) from 100mg lipid tissue, 조직, 셀, plant, and body fluids using columns and MagZol reagent |
| 응용 | RT-PCR, qRT-PCR, Northern hybridization, second-generation sequencing |
| 정제방법 | 미니 스핀 컬럼 |
| 정제기술 | 실리카 기술, acid phenol/guanidine extraction technology (MagZol pretreatment technology) |
| 가공방법 | 수동 (원심분리 또는 진공) |
| 샘플 유형 | Animal tissue, muscle fiber-containing tissue, adipose tissue, 배양된 세포, lymphocytes, simple plants, and other biological samples |
| 샘플량 | Animal tissue sample: 1-60mg (spleen ≤ 10mg) Cultured cells: 5 엑스 106 Plant leaves: 50-100mg |
| Yield | 2-200μg |
| 용출량 | ≥50μl |
| 실행당 시간 | ≤30 minutes（1-24 samples） |
| 컬럼당 액체 운반량 | 800μl |
| 컬럼의 결합 수율 | 100µg |
원칙
Tissue samples (10-100mg) are homogenized in MagZol Reagent. After the addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions. The sample is then applied to the spin column, where the total RNA (up to 100µg) binds to the membrane, and phenol and other contaminants are efficiently washed away. High-quality RNA is then eluted in 30-100µl of RNase-free water.
장점
- Efficient DNA removal – One-step RNA extraction can effectively remove genomic DNA
- High quality – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- 빠른 – 여러 샘플을 추출할 수 있습니다. 30 분
- High applicability – samples including animals, 식물, 박테리아, 세포, 등.
- High output – enables to processing of large samples and the yield can be up to 200μg
키트 내용물
| 내용물 | R413002 | D413003 |
| 정화 시간 | 50 준비 | 250 준비 |
| HiPure RNA Mini Columns | 50 | 250 |
| 2ml 수집 튜브 | 50 | 250 |
| MagZol 시약 | 60 밀리리터 | 270 밀리리터 |
| Buffer RW1 | 50 밀리리터 | 200 밀리리터 |
| Buffer RW2* | 20 밀리리터 | 50 밀리리터 |
| RNase Free Water | 10 밀리리터 | 30 밀리리터 |
보관 및 안정성
MagZol Reagent should be stored at 2-8°C upon arrival. 하지만, 단기 보관 (까지 12 주) 실온에서 (15-25℃) 성능에 영향을 미치지 않습니다. 나머지 키트 구성품은 실온에서 보관 가능 (15-25℃) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안.
실험 데이터
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