제품소개
This product offers a rapid and efficient method to isolate DNA from a wide range of samples, 포함:
- 조직
- 세포
- 피
- Saliva
- Swabs
- Blood Spots
- Semen
- And more!
The extracted DNA is ready for immediate use in downstream applications like:
- PCR (폴리 메라 제 연쇠 반응)
- qPCR (정량적 PCR)
- Southern Blotting
- Viral DNA detection
- Other DNA-based analyses
사양
| 특징 | 명세서 |
| 주요 기능 | Isolation of total DNA from tissue/blood /body fluid /swab /dry blood spots |
| 응용 | PCR,qPCR, Southern Bolt 및 바이러스 감지, 등. |
| 정제방법 | 미니 스핀 컬럼 |
| 정제기술 | 실리카 기술 |
| 가공방법 | 수동 (원심분리 또는 진공) |
| 샘플 유형 | 조직, 세포, 피, 타액, 면봉, 핏자국, 정액, 및 기타 임상 샘플 |
| 샘플량 | 고형 조직:1-10mg, 항응고제 혈액:200중 |
| Yield | 1 -15μg |
| 용출량 | ≥20μl |
| 실행당 시간 | 30 -60 분 |
| 컬럼당 액체 운반량 | 750μl |
| 컬럼의 결합 수율 | 100μg |
원칙
- 용해 및 소화: Begin by lysing and digesting the sample using a specialized lysate and protease combination. This step facilitates the release of DNA into the lysate, preparing it for the purification process.
- Adsorption Column: Transfer the lysate mixture to an adsorption column. 여기, the nucleic acid selectively adheres to the silica membrane, while proteins and other contaminants remain unadsorbed. This selective binding ensures the efficient removal of impurities.
- Filtration: Employ filtration to efficiently remove the unadsorbed proteins and other contaminants from the mixture. This step further enhances the purity of the nucleic acid sample.
- Thorough Washing: Perform a thorough washing step to eliminate any remaining proteins and impurities, ensuring the purity of the nucleic acid sample.
- 용출: 마지막으로, elute the purified nucleic acid from the adsorption column using a low-salt buffer solution (10mm 트리스, pH 9.0, 0.5mm EDTA). This gentle elution process preserves the integrity of the DNA, readying it for downstream applications.
장점
- 다재: Suitable for a diverse range of downstream applications, PCR을 포함하여, qPCR, 효소 소화, 이종 교잡, 그리고 더. Whether you’re performing basic research or advanced molecular assays, our DNA meets your requirements.
- 능률: Accelerate your workflow with fast DNA extraction. Say goodbye to time-consuming processes like leukocyte separation, 유기 추출, 또는 에탄올 침전. Our method streamlines the extraction process for swift results.
- Simplicity: Enjoy a straightforward extraction process. No complex steps or specialized equipment are required. Obtain all nucleic acids directly through simple digestion, saving you time and effort in the lab.
- Broad Applicability: Our DNA extraction method is versatile and adaptable. It can handle various sample types, including liquid samples, animal tissues, 및 배양된 세포. Whether you’re working with blood, 조직, or cell cultures, our method delivers consistent results across different sample types.
키트 내용물
| 내용물 | VD3018 |
| 정화 시간 | 100 |
| 2ml 수집 튜브 | 2×100 |
| 버퍼 ATL | 60 밀리리터 |
| 버퍼 AL | 60 중 |
| 버퍼 GW1 | 44 중 |
| 버퍼 GW2 | 50 중 |
| 단백질분해효소 K | 60 mg |
| 프로테아제 용해 완충액 | 5 밀리리터 |
| Buffer AF | 15 중 |
보관 및 안정성
- Proteinase K Storage:
- Upon arrival, 2~8°C에서 보관.
- 단기 보관 (까지 12 주) 실온에서 (15-25℃) is acceptable without affecting performance.
- Remaining Kit Components:
- Store at room temperature (15-25℃).
- Stable for at least 18 이런 조건에서 몇 달 동안.
- Entire Kit Storage:
- Can be stored at 2-8°C.
- Ensure buffers are redissolved before use if stored at this temperature.
- Buffers should be at room temperature when used.
실험 데이터
상품평
아직 상품평이 없습니다.