MagPure Blood DNA 프리캐스트 키트 (32/48 채널 머신)

소개

This product provides fast and easy methods for the purification of total DNA for reliable PCR 및 서던 블로팅. 총 DNA(예를 들어, 게놈의, 바이러스의, 미토콘드리아) can be purified from whole blood, 혈장, 혈청, 버피 코트, bone marrow, other body fluids, lymphocytes, 및 배양된 세포.

Relevant reagents were pre-packed in 96-well plates following the optimal protocol.

명세서

Features Specifications
Main Functions Isolation total DNA from 200μl whole blood
Applications PCR, Southern Bolt 및 바이러스 감지, 등
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Whole blood, concentrated blood, 버피 코트, and other samples
Sample amount 200μl
Elution volume ≥70μl
Time per run ≤40 minutes

원칙

This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA가 용해물로 방출됩니다.. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with a washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally, DNA was eluted by an Elution Buffer.

장점

• High binding force – suitable for handling DNA-rich samples, such as whole blood, 버피 코트, concentrated blood, 등.
• 빠른 – polydisperse magnetic beads, fast magnetic response, and short extraction time
• 고순도 – the obtained DNA can be directly used for second-generation sequencing, PCR-based detection, gene bank, 등.

키트 내용물

단백질분해효소 K, MagPureParticles should be stored at 2-8°C upon arrival. 하지만, 단기 보관(까지 24 주) 실온에서 (15-25℃) 성능에 영향을 미치지 않습니다. 나머지 키트 구성품은 실온에서 보관 가능 (15-25℃)그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안.