소개
- Extracts DNA from various sources: 조직, 세포, 피, 타액, 면봉, 핏자국, 정액, 및 기타 임상 샘플.
- Fast and easy to use.
- Purified DNA can be used for PCR, qPCR, 서던 블롯, viral DNA testing, 그리고 더.
명세서
| 특징 | 명세서 |
| 주요 기능 | Isolation of total DNA from 1-100ul blood, FFPE, 조직, and other samples |
| 응용 | Second-generation sequencing, PCR, 실시간 PCR, 등. |
| 정제방법 | Monodisperse magnetic beads |
| 정제기술 | 마그네틱 비드 기술 |
| 가공방법 | 수동 또는 자동 |
| 샘플 유형 | 항응고제 혈액, concentrated blood, 버피 코트, lymphocytes, 및 배양된 세포 |
| 샘플량 | Body fluid : 10 – 200μl, 조직 : ≤10mg |
| Yield | 10ng – 15μg |
| 용출량 | 80 -100μl |
| 실행당 시간 |
원칙
- Utilizes high-binding magnetic particles for purification, ensuring efficient DNA isolation.
- The sample undergoes lysis and digestion facilitated by lysate and Protease, DNA를 용액에 방출.
- Magnetic particles and binding solution are introduced, causing DNA to adhere to particle surfaces while impurities such as proteins are left unbound.
- Wash with a specialized solution to remove proteins and impurities, followed by ethanol wash to eliminate salts.
- DNA is ultimately eluted using an Elution Buffer, providing purified DNA ready for downstream applications.
Advantage
- High binding capability – ideal for processing DNA-rich samples like whole blood, 버피 코트, and concentrated blood.
- Exceptional purity – features carboxyl magnetic beads with minimal surface adsorption, ensuring superior purity.
- Versatile – applicable across a wide range of clinical samples, offering general utility.
키트 내용물
| 내용물 | IVD3101 |
| 정화 시간 | 200 |
| MagBind Particles | 4.5 밀리리터 |
| 단백질분해효소 K | 90 mg |
| 프로테아제 용해 완충액 | 10 밀리리터 |
| 버퍼 ATL | 60 밀리리터 |
| 버퍼 AL | 60 밀리리터 |
| Buffer BD* | 20 밀리리터 |
| Buffer BXW1* | 110 밀리리터 |
| 용출 버퍼 | 30 밀리리터 |
보관 및 안정성
- Proteinase K and MagBind Particles should be stored at 2–8°C upon receipt.
- 단기 보관 (까지 18 주) 실온에서 (15-25°C) does not compromise their performance.
- The remaining components can be stored dry at room temperature (15-25°C) 그리고 적어도 안정적으로 유지 18 이런 조건에서 몇 달 동안.
