Fast and Easy Total DNA 정제
- Objective: Reliable PCR and Southern Blotting
- 특징:
- Swift purification methods
- Easy-to-follow protocols
- 이익:
- Saves time and effort
- Ensures reliable PCR and Southern blotting results
- Applicability:
- Purifies various types of total DNA:
- Genomic
- Viral
- Mitochondrial
- Suitable for diverse sample types:
- 전혈
- 혈장
- 혈청
- Buffy coat
- Bone marrow
- Other body fluids
- Lymphocytes
- Cultured cells
- Purifies various types of total DNA:
명세서
| 특징 | 명세서 |
| 주요 기능 | Isolation of total DNA from 200μl whole blood |
| 응용 | PCR, Southern Bolt 및 바이러스 감지, 등 |
| 정제기술 | 마그네틱 비드 기술 |
| 가공방법 | 수동 또는 자동 |
| 샘플 유형 | 전혈, concentrated blood, 버피 코트, and other samples |
| 샘플량 | 200μl |
| 용출량 | ≥70μl |
| 실행당 시간 | ≤40 minutes |
원칙
- Sample lysis and digestion facilitated by lysate and Protease.
- DNA가 용해물로 방출됩니다..
- DNA adsorption onto magnetic particles’ surface, excluding impurities like proteins.
절차:
- Sample lysis and digestion: Lysate and Protease act on the sample.
- DNA release: DNA가 용해물로 방출됩니다..
- DNA adsorption: Magnetic particles and binding solution are added, facilitating DNA adsorption.
- Impurity removal: Proteins and impurities are removed without DNA adsorption.
- 세탁:
- 첫 번째, wash with washing solution to remove proteins and impurities.
- Ethanol wash to eliminate salts.
- 용출: DNA is eluted using an Elution Buffer.
장점:
- High specificity: Selective DNA binding, excluding impurities.
- 능률: Rapid purification process.
- Minimal sample loss: Enhanced DNA recovery.
응용:
- Ideal for PCR and Southern blotting experiments requiring purified DNA.
- Suitable for various sample types and DNA sources.
장점
- High Binding Force: Magnetic beads with exceptional binding capacity efficiently capture DNA molecules even from samples with abundant DNA, such as whole blood, 버피 코트, and concentrated blood.
- Rapid Processing: The use of polydisperse magnetic beads ensures a fast magnetic response, leading to significantly reduced extraction times.
- High-Purity DNA: The optimized protocol delivers highly purified DNA, eliminating the need for further purification steps. This high-quality DNA is directly usable for various downstream applications:
- Second-generation sequencing (NGS)
- PCR-based detection methods
- Gene bank construction
- And other DNA analyses requiring high purity
키트 내용물
| 내용물 | D631001 | D631002 | D631003 |
| 정화 시간 | 48 | 96 | 480 |
| MagPure 입자 | 1.7 밀리리터 | 3.5 밀리리터 | 17 밀리리터 |
| 단백질분해효소 K | 24 mg | 50 mg | 220 mg |
| 프로테아제 용해 완충액 | 1.8 밀리리터 | 3 밀리리터 | 15 밀리리터 |
| Buffer MLA | 30 밀리리터 | 60 밀리리터 | 270 밀리리터 |
| Buffer DW1 | 60 밀리리터 | 120 밀리리터 | 550 밀리리터 |
| Buffer CW | 30 밀리리터 | 60 밀리리터 | 270 밀리리터 |
| Buffer BW3 | 30 밀리리터 | 60 밀리리터 | 270 밀리리터 |
| 용출 버퍼 | 15 밀리리터 | 30 밀리리터 | 100 밀리리터 |
보관 및 안정성
단백질분해효소 K, MagPureParticles should be stored at 2-8°C upon arrival. 하지만, 단기 보관(까지 24 주) 실온에서 (15-25℃) 성능에 영향을 미치지 않습니다. 나머지 키트 구성품은 실온에서 보관 가능 (15-25℃)그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안.
