소개
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples(including serum and plasma). The kit is based on super paramagnetic particles purification technology. Purified DNA/RNA is ready for downstream applications such as Real Time PCR, biochip analysis, NGS and other related experiments.
세부
명세서
| 특징 | 명세서 |
| 주요 기능 | Extract Pathogen RNA/DNA from 0.5-1.5ml whole blood, 혈장, 혈청, 체액, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for mNGS/tNGS application, remove host background nucleic acid. |
| 응용 | Real Time PCR, biochip analysis, NGS |
| 제품 | Pathogen DNA / RNA |
| 정제방법 | Polydisperse magnetic beads |
| 정제기술 | 마그네틱 비드 기술 |
| 가공방법 | 수동 또는 자동 |
| 샘플 유형 | whole blood, 혈장, 혈청, 체액, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution |
| 샘플량 | 0.5 – 1.5 밀리리터 |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
원칙
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
키트 내용물
| 내용물 | R667200C | R6672-02C |
| 정화 시간 | 24 준비 | 96 준비 |
| 2ml Bead Tube | 24 | 96 |
| Buffer CLB2 | 10 밀리리터 | 40 밀리리터 |
| 단백질분해효소 K | 12 mg | 50 mg |
| 프로테아제 용해 완충액 | 1.8 밀리리터 | 6 밀리리터 |
| 완충기 SDS | 1.5 밀리리터 | 10 밀리리터 |
| 시약 DX | 1.0 밀리리터 | 3.0 밀리리터 |
| Nuclease | 25 KU | |
| Nuclease Buffer | 1.5 밀리리터 | 5.0 밀리리터 |
| MagBind Particles | 1.0 밀리리터 | 3.5 밀리리터 |
| Buffer MLB | 15 밀리리터 | 60 밀리리터 |
| Buffer MW1* | 13 밀리리터 | 44 밀리리터 |
| 버퍼 MW2* | 10 밀리리터 | 50 밀리리터 |
| Buffer AVE | 10 밀리리터 | 20 밀리리터 |
보관 및 안정성
MagBind Particles, Nuclease and Proteinase K should be stored at 2–8°C upon arrival. 하지만, 단기 보관 (까지 8 주) 실온에서 (15-25°C) 성능에 영향을 미치지 않습니다. 나머지 키트 구성품은 실온에서 보관 가능 (15-25°C) and are stable for 18 이런 조건에서 몇 달 동안.
상품평
아직 상품평이 없습니다.