소개
This product is suitable for extracting total pathogen RNA from biological samples with no/low cell content such as body fluid, 혈청, 혈장, soaking solution, tissue homogenate supernatant, culture medium supernatant, 등. The purified RNA can be used for clinical in vitro detection.
It is special designed for Pathogen RNA extraction from Sputum samples, can be used in tuberculosis (TB) 발각.
세부
명세서
특징 | 명세서 |
Main Function | Extract total pathogen RNA from cell-free/low-content cell biological samples such as body fluids, 혈청, 혈장, tissue homogenate supernatant, special design for sputum samples. Used in tuberculosis detection. |
응용 | RT-PCR,PCR |
제품 | Pathogen RNA |
정제방법 | Polydisperse magnetic beads |
정제기술 | 마그네틱 비드 기술 |
가공방법 | 수동 또는 자동 |
샘플 유형 | cell-free/low-content cell biological samples such as body fluids, 혈청, 혈장, tissue homogenate supernatant, 담 |
샘플량 | 200-300μl |
Adaptive instrument | Nucleic acid extractor, pipetting workstation |
원칙
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
장점
- 빠른 – 여러 샘플을 추출할 수 있습니다. 40 minutes by column method
- High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
- Safe – no phenol chloroform extraction required
- Sensitive – DNA/RNA can be recovered at the level of PG
키트 내용물
내용물 | IVD6672C |
정화 시간 | 200 준비 |
2ml Bead Tube | 4 엑스 50 |
MagPure Particle | 7.0 밀리리터 |
단백질분해효소 K | 100 mg |
프로테아제 용해 완충액 | 6 밀리리터 |
DTT (Powder) | 2g |
완충기 SDS | 15 밀리리터 |
Buffer MLBN | 220 밀리리터 |
DNase I | 4 엑스 0.6 밀리리터 |
DNase Buffer | 60 밀리리터 |
Buffer MW1* | 53 밀리리터 |
버퍼 MW2* | 50 밀리리터 |
Buffer NFW | 30 밀리리터 |
보관 및 안정성
MagPure 입자 and Proteinase K, DNase I should be stored at 2–8°C upon arrival. 하지만, 단기 보관 (까지 2 주) 실온에서 (15-25°C) 성능에 영향을 미치지 않습니다. 나머지 키트 구성품은 실온에서 보관 가능 (15-25°C) and are stable for 18 이런 조건에서 몇 달 동안.
상품평
아직 상품평이 없습니다.