This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 분. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5×106 배양된 세포, 20mg tissue and <50mg plant samples.
세부
명세서
| 특징 | 명세서 |
| 주요 기능 | Isolation total RNA from 50mg plant using magnetic particles |
| 응용 | RT-PCR, cDNA 합성, second generation sequencing |
| 정제방법 | Polydisperse magnetic beads |
| 정제기술 | 마그네틱 비드 기술 |
| 가공방법 | 수동 또는 자동 |
| 샘플 유형 | Plant and fungus samples |
| 샘플량 | ≤50mg |
| Yield | 2-100μg |
| 실행당 시간 | 60분 이하 |
원칙
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
장점
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Safe – no phenol chloroform extraction required
- 빠른 – 여러 샘플을 추출할 수 있습니다. 60 minutes by column method
- Universal – two lysates suitable for most plant or fungal tissue samples
키트 내용물
| 내용물 | R664101 | R664102 | R664103 |
| 정화 시간 | 48 준비 | 96 준비 | 480 준비 |
| MagPure RNA Particles | 1.7 밀리리터 | 3.5 밀리리터 | 18 밀리리터 |
| DNase I | 600 μl | 2 엑스 600 μl | 10 엑스 600 μl |
| DNase Buffer | 30 밀리리터 | 40 밀리리터 | 200 밀리리터 |
| Buffer PRC1 | 40 밀리리터 | 70 밀리리터 | 350 밀리리터 |
| Buffer RL | 40 밀리리터 | 70 밀리리터 | 350 밀리리터 |
| Buffer MCB* | 18 밀리리터 | 30 밀리리터 | 150 밀리리터 |
| Buffer MW1* | 22 밀리리터 | 44 밀리리터 | 220 밀리리터 |
| 버퍼 MW2* | 20 밀리리터 | 50 밀리리터 | 2 엑스 100 밀리리터 |
| RNase Free Water | 10 밀리리터 | 15 밀리리터 | 120 밀리리터 |
보관 및 안정성
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. 하지만, 단기 보관 (DNase I up to 1 주, MagPure RNA Particles up to 8 주) 실온에서 (15-25°C) 성능에 영향을 미치지 않습니다. 나머지 키트 구성품은 실온에서 보관 가능 (15-25°C) 그리고 적어도 안정적이다 18 이러한 조건 하에서 개월.
상품평
아직 상품평이 없습니다.