MagPure 플라스미드 미니 키트

소개

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy 플라스미드 DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR 확대.

세부

명세서

원칙

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA가 용해물로 방출됩니다.. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

장점

• 고순도 – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, 등.
• 높은 수율 – up to 20μg plasmid can be binded in one column

키트 내용물

보관 및 안정성

RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. 하지만, 단기 보관 (까지 24 주) 실온에서 (15-25℃) 성능에 영향을 미치지 않습니다. 나머지 키트 구성품은 실온에서 건조한 상태로 보관할 수 있습니다. (15-25℃) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.

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