소개
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, 노던 블로팅, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
세부
명세서
| 특징 | 명세서 |
| 주요 기능 | Isolation miRNA from 0.3-0.5ml serum or plasma using magnetic particles |
| 응용 | RT-PCR, cDNA 합성, second generation sequencing |
| 정제방법 | Polydisperse magnetic beads |
| 정제기술 | 마그네틱 비드 기술 |
| 가공방법 | 수동 또는 자동 |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| 샘플 유형 | 혈청, 혈장, acellular samples |
| 샘플량 | 300μl |
원칙
This product is based on the purification method of high binding magnetic particles. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
장점
- Safe – no phenol chloroform extraction and no use of Trizol reagent
- 빠른 – 여러 샘플을 추출할 수 있습니다. 60 minutes by column method
키트 내용물
| 내용물 | R662801 | R662802 | R662803 |
| 정화 시간 | 48 준비 | 96 준비 | 5 엑스 96 준비 |
| MagPure Particles N | 1.7 밀리리터 | 3.5 밀리리터 | 17 밀리리터 |
| Buffer CFL | 6 밀리리터 | 12 밀리리터 | 60 밀리리터 |
| Buffer CPL | 1.8 밀리리터 | 3.5 밀리리터 | 20 밀리리터 |
| Buffer MGW1* | 30 밀리리터 | 60 밀리리터 | 250 밀리리터 |
| 버퍼 MW2* | 12 밀리리터 | 25 밀리리터 | 100 밀리리터 |
| RNase Free Water | 10 밀리리터 | 20 밀리리터 | 60 밀리리터 |
보관 및 안정성
MagPure Particle N should be stored at 2–8°C upon arrival. 하지만, 단기 보관 (까지 8 주) 실온에서 (15-25°C) 성능에 영향을 미치지 않습니다. 나머지 키트 구성품은 실온에서 보관 가능 (15-25°C) 그리고 적어도 안정적이다 18 이런 조건에서 몇 달 동안.
상품평
아직 상품평이 없습니다.