소개
This product is suitable for extracting total viral nucleic acid from cell-free/low-content cell biological samples such as body fluids, 혈청, 혈장, soaking solutions, tissue homogenate supernatant, and culture supernatant. The Purified DNA/RNA is used for RT-PCR and PCR detection.
세부
명세서
| 특징 | 명세서 |
| 주요 기능 | Extract viral DNA/RNA from 200μl cell-free samples by magnetic beads |
| 응용 | RT-PCR，PCR，NGS |
| 제품 | Viral DNA / RNA |
| 정제방법 | Polydisperse magnetic beads |
| 정제기술 | 마그네틱 비드 기술 |
| 가공방법 | 수동 또는 자동 |
| 샘플 유형 | cell-free/low-content cell biological samples such as body fluids, 혈청, 혈장, tissue homogenate supernatant |
| 샘플량 | 200μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
원칙
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA/RNA is released into the lysate. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Nuclease Free Water.
장점
- 빠른 – 여러 샘플을 추출할 수 있습니다. 40 minutes by column method
- High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
- Safe – no phenol chloroform extraction required
- Sensitive – DNA/RNA can be recovered at the level of PG
키트 내용물
| 내용물 | IVD5412 |
| 정화 시간 | 200 준비 |
| MagPure Particles N | 5 밀리리터 |
| PK/캐리어 RNA | 50 mg |
| Protease Dissolve Buffer Blue | 5 밀리리터 |
| Buffer MLB* | 120 밀리리터 |
| Buffer MW1* | 53 밀리리터 |
| RNase Free Water | 30 밀리리터 |
보관 및 안정성
This kit is shipped and stored at room temperature and is valid for 12 개월.
실험 데이터
상품평
아직 상품평이 없습니다.