이 상품은 얼음주머니로 포장하여 FedEx 또는 UPS를 통해 배송해야 합니다..
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소개
RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5′-ribose of a nucleotide and the phosphategroup attached to the 3′-인접한 피리미딘 뉴클레오티드의 리보스. The resulting 2′,3′-cyclic phosphate is hydrolyzed to the corresponding 3′-뉴클레오시드 인산염. The highest activity is exhibited with single-stranded RNA. RNase A is a single chain polypeptide containing 4 이황화물 다리.
A major application for RNase A is the removal of RNA from preparations of 플라스미드 DNA. The enzyme is active under a wide range of reaction conditions. 낮은 염분 농도에서 (0 에게 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well as the RNA strand in RNA-DNA hybrids. 하지만, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.
세부
명세서
| 특징 | 명세서 |
| 청정 | ≥60% RNase A basis (SDS-페이지) |
| Enzymatic activity | >50 쿠니츠 단위/mg 단백질 |
| Optimum reaction temperature | 60℃ (effective active temperature is 15-70°C) |
| Transportation conditions | Normal temperature transportation |
| 보존 조건 | -20-8℃, dry storage, long-term storage should be placed at -20°C. |
| 애플리케이션 1: adding in the extraction process | 1. Plasmid Extraction: add RNase A (25mg/ml) to buffer P1 with a final concentration of 100-300μg/ml. 2. DNA 추출: add RNase A (25mg/ml) to the digestion solution with a final concentration is 100-400μg/ml, mix well and place at room temperature for 10-15 분. when SDS/CTAB in lysate exceeds 2%, RNase activity will be significantly inhibited; Guanidine salt(>4M guanidine hydrochloride or >3M guanidine isothiocyanate) also significantly inhibited RNase A. When RNase A is added to the lysate, the RNase digestion effect can be extracted by appropriately diluting to reduce the concentration of SDS, CTAB and guanidine salt. |
| 애플리케이션 2: | 1. Remove RNA contamination from crude genomic DNA products: add DNase free RNase A (10mg/ml) to crude DNA products with a final concentration of 10-100μg/ml. After mixing, place at room temperature for 10 분. 2. Remove RNA contamination from plasmid DNA products: add DNase free RNase A (10mg/ml) to crude DNA products with a final concentration of 10μg/ml. After mixing, it can be directly used for sequencing at room temperature for 10 분. |
주문정보
| 내용물 | C12123 | C12124 | C12128 | C12129 |
| RNase A Solution (25mg/ml) | 10 밀리리터 | 100 밀리리터 | ||
| DNase Free RNase A Solution (10mg/ml) | 10 밀리리터 | 100 밀리리터 |
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