솔라바이오 다이렉트 빌리루빈 (DBIL) 분석 키트 50T | 48에스

메모: 테스트 전에 예측을 위해 2~3개의 서로 다른 샘플을 채취합니다..

운영장비: 분광 광도계/마이크로플레이트 리더

카탈로그 번호: BC5175

크기: 50티 | 48에스

구성요소:

시약 I: 25 밀리리터×1. 2~8℃에서 보관.

시약 II: 6 밀리리터×1. 2~8℃에서 보관.

제품 설명：

직접 빌리루빈 (DBIL) is also called conjugated bilirubin. After indirect bilirubin enters the liver, it is combined with glucuronic acid by the action of glucuronosyltransferase in the liver. The increase of direct bilirubin is of great significance for clinical diagnosis of obstructive jaundice, hepatocellular jaundice, liver cancer, pancreatic head cancer, cholelithiasis, and cholangiocarcinoma. Direct bilirubin could be oxidized by sodium nitrite to form biliverdin, which has an absorbance of 450 nm. The content of direct bilirubin can be calculated by detecting the wavelength change at 450 nm.

필요하지만 제공되지 않는 시약 및 장비：

분광 광도계/마이크로플레이트 리더, 책상 원심분리기, constant temperature foster box/water bath, pipette, 마이크로 유리 큐벳/96웰 평면 바닥 플레이트, 얼음, 그리고 증류수.

절차

ㅏ. 샘플 준비:

혈청, 혈장, or other liquid samples: Detect the sample directly. If the solution is turbid, measure centrifuging.

비.결정 절차：

1. Preheat the spectrophotometer/microplate reader for 30min, 파장을 조정하여 450 nm, and set the spectrophotometer counter to zero with distilled
2. 결정:

씨.계산：

96 바닥이 평평한 접시

DBIL content (μmol/L) =1495.7×(ΔAT-ΔAB)-15.343

Micro glass 큐벳

DBIL content (μmol/L) =1099.7×(ΔAT-ΔAB)-10.73

메모:

1. Bilirubin decomposes easily in light. Avoid light during
2. If the ΔA is lower, it is recommended to increase the sample size before determination; If ΔA＞5, it is recommended to dilute the sample before determination.

Examples:

1. Take mouse serum to follow the determination procedure to operate. Determination with 96 바닥이 평평한 접시, and calculate ΔAT=A1T-A2T=0.217-0.170=0.047, ΔAB=A1B-A2B=0.044-0.044=0. The calculated content is as follows:

DBIL content (μmol/L) =1495.7×(ΔAT-ΔAB)-15.343=54.95 μmol/L.

관련 상품

BC5180/BC5185 Total bilirubin (TBIL) 콘텐츠 분석 키트