글리코겐 분석 키트

메모: 테스트 전에 예측을 위해 2~3개의 서로 다른 샘플을 채취합니다..

운영장비: Spectrophotometer/ microplate reader

카탈로그 번호: BC0345

크기:100T/96S

구성요소:

Extract reagent: 100밀리리터×1, 4℃에서 보관.

섭정 1세: 가루×1, storage at 4℃.10 mg of glucose, 추가하다 1 mL of distilled water to dissolve it before use. Diluted with distilled water to 0.1 mg/mL glucose solution for standby, ready to use. 0.1 mg/mL glucose standard solution, 4℃에서 보관.

Regent Ⅱ: 가루×1, 4℃에서 보관.

작업 솔루션: Pour 6 mL of distilled water into Reagent Ⅱ and slowly pour 24 mL of concentrated sulfuric acid. Dissolve and mix thoroughly before use. Unused reagents are valid at 4 °C for one week.

제품 설명

Glycogen is a high molecular polysaccharide composed of glucose units. It is one of the main storage forms of sugar. It is mainly stored in the liver and muscle as backup energy, and is called liver glycogen and muscle glycogen, 각기. Glycogen can regulate blood glucose concentration. Glycogen can be synthesized in the liver when blood glucose rises. When blood sugar decreases, liver glycogen is broken down into glucose to supplement blood sugar. 그러므로, liver glycogen is important to maintain the relative balance of blood sugar. Muscle glycogen is a form of glycogen storage in muscles. When lots of blood sugar is consumed during strenuous exercise, muscle glycogen cannot be broken down directly into blood sugar. It must first be broken down to produce lactic acid, which is circulated to the liver with the blood, and transformed into liver glycogen through glycogen glucose.

Determination principle: anthrone method. Glycogen is extracted with strong alkaline extract, and the glycogen content is measured using an anthrone method under strongly acidic conditions.

필요하지만 제공되지 않는 시약 및 장비.

에스pectrophotometer/ microplate reader, 책상 원심분리기, 이송 피펫, micro glass cuvette/96-well plate, mortar, concentrated sulfuric acid (H2SO4) 그리고 증류수.

절차:

나. Sample extraction:

1. Cells or bacteria: 모으다 5-10 million bacteria or cells into a centrifuge tube, discard the supernatant after centrifugation; add 0.75mL of extraction reagent to ultrasonically break bacteria or cells (힘 20% or 200W, ultrasonic 3s, 10s interval, 반복하다 30 타임스) ); Transfer to a 10mL tube, boil in a boiling water bath for 20min (close tightly to prevent water loss), shake the tube every 5 min to fully mix; take out the tube and cool, take up to 5mL with distilled water and mix. Centrifuge at 8000g and 25℃ for 10min, take the supernatant for testing.

2. 조직: Weigh 0.1~0.2g sample and put it in a 10 mL tube, 추가하다 0.75 mL of Extraction solution, boil in boiling water bath for 20 분 (close tightly to prevent water loss), shake the test tube every 5 min to fully mix. After all the tissue dissolved, take out the tube and cool down, then make up to 5 mL with distilled water. Centrifuge at 8000g and 25℃ for 10 분, take the supernatant for testing.

II. 결정 절차:

1. Preheat the 에스pectrophotometer/ microplate reader for 30 분, 파장을 조정하다 620 nm, 증류수로 0을 설정하다
2. Sampling table (add the following regents in EPtube)

잘 섞다, place in a boiling water bath for 10 분 (close tightly to prevent water loss), cool, and take 200 μL into micro glass cuvette/96-well plate to read the absorbance of the blank tube, standard tube, and measurement tube at 620 nm, and record them as A1, A2, and A3. The blank tube and standard tube need only be tested once.

III. 계산:

1. 샘플 중량

Sorbitol (mg/g fresh weight) =(Cs×V1)×(A3-A1)¶(A2-A1)¶(W×V1÷V2)÷1.11

= 0.450×(A3-A1)÷（A2-A1)¼W

2. 단백질 농도

Sorbitol (mg/mg 프로트) = (Cs×V1)×(A3-A1) ¶(A2-A1) ¶(V1×Cpr) ÷1.11

=0.09×(A3-A1) ÷（A2-A1) ¼Cpr

3. The number of bacteria or cells:

Sorbitol（mg/104 cell）= (Cs×V1)×(A3-A1)¶(A2-A1)¶(number of bacteria or cells×V1÷V2) ÷1.11

= 0.450×(A3-A1)÷（A2-A1)÷number of bacteria or cells

1.11: It is a constant that glucose content converted to glycogen content, That is, the color of 111 μg of glucose with anthrone reagent is equivalent to that of 100 μg of glycogen with anthrone reagent.

CS: the concentration of standard, 0.1 mg/mL V1: 샘플량, 0.06 밀리리터;

V2: Total sample volume, 5 밀리리터;

심폐소생술: 샘플 단백질 농도, mg/mL; 여: 샘플 중량, g

Number of bacteria or cells: 104 as the unit, ten thousand

메모:

If A is greater than 1.4, dilute the sample with distilled water and multiply it by the corresponding dilution factor in the calculation formula.

관련 상품

BC2450/BC2455 Plant Tissue Fructose Content Assay Kit

BC2540/BC2545 Cellulase(씨엘) 활동 분석 키트

BC0330/BC0335 Trehalose Content Assay Kit

BC2510/BC2515 Trehalase Activity Assay Kit

BC2520/BC2525 Sorbitol Content Assay Kit

BC2530/BC2535 Sorbitol Dehydrogenase(SDH) 활동 분석 키트

BC0230/BC0235 Reducing Sugar(RS) 콘텐츠 분석 키트

BC2490/BC2495 Blood Glucose Content Assay Kit

Technical Specification:

검출 한계: 0.002 mg/mL

The linear range: 0.003125-0.25 mg/mL