사양
| 기인하다 | 세부 |
|---|---|
| 저장 | 2-8℃ |
| 단위 | Box |
| 유효기간 | 6 개월 |
| 영어 이름 | Pepsase Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| 사양 | 100T/48S |
Product Composition
| 시약 이름 | 사양 | Storage Condition |
|---|---|---|
| 추출 용액 | 액체 50 밀리리터×1병 | 4℃에서 보관 |
| 시약 1 | Powder×1 bottle | 4℃에서 보관 |
| 시약 2 | 액체 15 밀리리터×1병 | 4℃에서 보관 |
| 시약 3 | Powder×1 bottle | 4℃에서 보관 |
Instructions for preparing the solutions:
- Solution One: 추가하다 10 mL of Reagent Two before use and dissolve completely.
- Solution Three: 추가하다 10 mL of distilled water before use and dissolve completely.
제품 설명:
- Pepsin is secreted by the chief cells of the gastric mucosa and hydrolyzes proteins in food into small peptides. It is commonly used for the identification of hypochlorhydria, and reduced secretion of pepsin may also occur in conditions such as chronic gastritis, chronic gastric dilation, and chronic duodenitis.
- Pepsin catalyzes the hydrolysis of hemoglobin, and the hydrolysis product tyrosine exhibits a characteristic absorption peak at 275nm. Enzyme activity is calculated by measuring the changes in absorbance.
Instruments and supplies required:
- UV spectrophotometer/enzyme marker
- Benchtop centrifuge
- Water bath
- 조정 가능한 피펫
- Micro quartz cuvette/96-well UV plate
- Mortar and pestle
- Ice
- 증류수
메모: It is recommended to perform a pilot experiment with 2-3 samples showing significant expected differences before conducting the actual experiment. If the absorbance values of the samples are not within the measurement range, dilution or increasing the sample volume for detection is recommended.
상품평
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