- 정량적 PCR (qPCR) Overview:
- qPCR, also known as Real-time PCR, is a widely used technology for precise analysis of gene expression.
- It can be categorized into dye-based and probe-based methods, with dye-based being more popular, convenient, and cost-effective.
- Dye-based qPCR monitors real-time fluorescence of the dye binding to double-stranded DNA to measure DNA amplification indirectly during each cycle.
- Ct 값 (Cq 값) is determined when the fluorescence signal is distinctly detected over the background, which can be used to evaluate target abundance or calculate absolute quantities.
- SYBR Green 2xqPCR Mix Master Mix:
- Provides superior specificity, 강력한 증폭 효율, ideal reproducibility, and stability in quantifying target DNA or cDNA.
- It’s a 2X premix containing hot-start Taq 중합효소 combined with an antibody for preferable specificity and high amplification speed.
- SYBR Green I in the mix emits fluorescence when bound to double-stranded DNA amplified in each cycle, allowing for indirect quantification of amplification products in real-time.
- Use SYBR or SYBR/FAM scan mode setting on the real-time instrument.
- Limitations of Dye-based qPCR:
- SYBR Green I은 모든 이중 가닥 DNA에 삽입 가능, including primer-dimers or other undesired products, causing nonspecific fluorescence.
- To confirm specificity, perform a melt curve analysis after amplification, where a sharp peak around the annealing temperature is ideal.
SYBR 그린 2xqPCR 믹스 1ml *10
$80.00
배송비(USD) 45 - USD 이상 무료 300DTE는 분자 테스트 온라인 판매를 전문으로 하는 중국 기반의 전자상거래 플랫폼입니다., 엘리사, 및 관련 제품.
- 제조업체: 중국 대표 브랜드
- 배송: 공장에서 직접 FedEx 신속 배송
- 이내에 반품 또는 교환이 가능합니다. 30 날
- 결제 방법: 안전한 PayPal 또는 신용카드.
설명
추가 정보
| 무게 | 0.7 킬로그램 |
|---|---|
| 상표명 |
상품평
아직 상품평이 없습니다.