Introduction
- Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease that acts on the phosphodiester bond, particularly those adjacent to pyrimidine nucleosides.
- It produces polynucleotides with a free hydroxyl group at the 3′ end and a phosphate group at the 5′ end.
- The optimum pH for DNase activity is 7.8.
- DNase activity is enhanced by divalent metals and inhibited by chelating agents such as EDTA and sodium dodecyl sulfate (SDS).
- 5 mM calcium ions can serve as stabilizers, protecting DNase from degradation by hydrolases.
- This product is extracted from bovine pancreas and purified using chromatography to remove contamination from other hydrolases.
Specifications
| Features | Specifications |
| Molecular weight | 32KDa |
| Isoelectric point | 8.9 |
| pH range | 4.0 – 10.0 |
| Specific activity | 3000 Kunitz units/mg |
| Preservation conditions | It is recommended to store at -20°C to ensure the stability of activity to the greatest extent (normal temperature transportation or storage will not reduce enzyme activity). |
| Solution | 50mM Tris (pH7.4)，10mM CaCl2，50% (v/v) glycerol |
| Recommended application | Remove DNA |
| RNase Residue | Not detection |
| RNase-Free DNase I Prepare | Dissolve DNase I at 1 mg/ml in 0.1M iodoacetic acid plus 0.15M sodium acetate at a final pH of 5.3. The solution is then heated 40-60 minutes at 55°C and cooled. Finally, 1M CaCl2 is added to the solution to 5mM. Store frozen in small aliquots. |
Advantages
- Derived from a bovine pancreas, this protein offers a pure natural source.
- Demonstrates high activity and purity, boasting over 3000 Kunitz units per milligram.
- Offers excellent cost-performance ratio, delivering exceptional value for investment.
Ordering information
| Contents | C12131 | C12132 |
| Dnase I, Lyophilizate, >3000 units/mg protein | 100 mg/Bottle | 1 g/Bottle |
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