HiPure Circulating Nucleic Acid Micro Kit

HiPure Circulating Nucleic Acid Micro Kit, is designed to efficiently purify short nucleic acids (<1000bp) from human biological fluids.

• Target Molecules:
  • Tumor-derived cell-free DNA fragments (cfDNA) and mRNA in blood
  • Fetal nucleic acids in maternal blood
  • Free-circulating cell-free RNA (cfRNA)
  • Viral nucleic acids
• Sample Compatibility:
  • Human plasma
  • Human serum
  • Human urine
  • Samples can be fresh or frozen (once only)
• Efficient Purification: The kit isolates short nucleic acids present in biofluids.
• High Purity: Purified nucleic acids are free of proteins, nucleases, and other contaminants.
• Downstream Applications: The eluted nucleic acids are ready for use in various downstream analyses, including:
  • Amplification reactions (e.g., PCR)
  • Long-term storage (-30°C to -15°C)

Benefits:

• Suitable for Short Nucleic Acids: Specifically designed to capture short fragments often found in biofluids.
• Broad Sample Compatibility: Works effectively with various human biofluids.
• High-Quality Results: Delivers purified nucleic acids free of impurities, ensuring reliable downstream applications.
• Convenient Storage: Purified nucleic acids can be stored for long-term use.

This kit empowers researchers studying circulating nucleic acids in various fields, including oncology, non-invasive prenatal testing (NIPT), and viral diagnostics.

Specifications

Principle

This product offers a fast and efficient method for purifying DNA using silica column chromatography. Here’s a breakdown of the process:

1. Lysis and Digestion:

• The sample is treated with a lysate solution and protease to break open cells and degrade proteins. This releases DNA into the solution.

2. Binding to Silica Membrane:

• The lysate containing the DNA is transferred to an adsorption plate or filtration column. The silica membrane in these tools selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.

3. Washing:

• Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.

4. Elution:

• A low-salt buffer is used to elute the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing collection of purified DNA in a separate tube.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis, such as PCR, Southern blotting, and next-generation sequencing.

Advantages

• Efficient Purification: Silica column technology offers a streamlined process for isolating DNA from complex biological samples.
• High-Quality DNA: The selective binding properties of the silica membrane ensure the purification of high-purity DNA, suitable for various downstream applications.
• Simplified Workflow: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.

Kit Contents

Storage and Stability

Optimal Performance:

• Store Proteinase K and Carrier RNA at 2-8°C (refrigerator) upon arrival for best results.

Acceptable Short-Term Storage:

• Proteinase K and Carrier RNA can be stored for up to 12 weeks at room temperature (15-25°C) without affecting their performance.

Remaining Kit Components:

• Store all other components of the kit dry at room temperature (15-25°C).
• They are stable for at least 18 months under these conditions.

Important Notes:

• If storing the entire kit at 2-8°C, ensure buffers are redissolved before use.
• Always warm all buffers to room temperature before using them for optimal performance.

By following these storage guidelines, you can ensure the longevity and effectiveness of your DNA extraction kit.