HiPure Circulating DNA Mini Kit, designed to efficiently purify short, cell-free DNA (cfDNA) molecules from human biological fluids:

• Target:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Sample Compatibility:
  • Human plasma
  • Human serum
  • Human urine
  • Samples can be fresh or frozen (once only)
• Focus on Short DNA: The kit isolates short nucleic acids (<1000bp) commonly found in biofluids.
• Efficient Purification: The protocol allows for effective recovery of cfDNA from various biofluids.
• Ready for Downstream Use: Purified cfDNA is suitable for diverse applications, including:
  • PCR and other amplification reactions
  • Next-generation sequencing (NGS) analyses

Benefits:

• Optimized for Short cfDNA: Specifically designed to capture short DNA fragments relevant for biomarker discovery and liquid biopsies.
• Broad Sample Compatibility: Works effectively with various human biofluids, offering flexibility in sample selection.
• Streamlined Workflow: The miniaturized kit format allows for faster processing compared to larger kits.

This kit empowers researchers to study circulating DNA in various fields, including oncology, non-invasive prenatal testing (NIPT), and liquid biopsy applications.

Specifications

Principle

This product utilizes silica gel columns for a rapid and reliable DNA purification process.

1. Lysis and Digestion: The sample undergoes treatment with a lysate solution and protease. This breaks open cells and degrades proteins, releasing DNA into the solution.

2. Binding to Silica Membrane: The lysate containing the DNA is transferred to an adsorption plate or filtration column. The silica membrane in these tools selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.

3. Washing: Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.

4. Elution: A low-salt buffer (typically 10 mM Tris, pH 8.0) is used to elute the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis, such as PCR, Southern blotting, and next-generation sequencing.

Advantages

• High yield – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
• High concentration – low elution volume, ensuring high nucleic acid concentration
• High purity – low alcohol binding method, completely removing inhibitor and protein pollution
• High recovery – DNA can be recovered at the level of PG by silica gel column purification

Kit Contents

Storage and Stability

Here’s a quick guide on storing your DNA extraction kit to ensure it functions at its best:

Ideal Storage:

• Store Proteinase K and Carrier RNA in the refrigerator (2-8°C) upon arrival for maximum effectiveness.

Acceptable Short-Term Storage:

• Proteinase K and Carrier RNA can be kept at room temperature (15-25°C) for up to 12 weeks without impacting their performance.

Storing Remaining Components:

• All other kit components should be stored dry at room temperature (15-25°C).
• They will remain stable for at least 18 months under these conditions.

Important Notes:

• If storing the entire kit at 2-8°C, remember to redissolve buffers before use.
• Always ensure all buffers reach room temperature before using them for optimal results.

By following these storage recommendations, you can guarantee your DNA extraction kit is always ready for use and delivers the best possible results.