HiPure Plasmid Mini Kit

Introduction

• Fast, Simple, and Cost-Effective: Offers a rapid, easy-to-use, and economical method for plasmid DNA miniprep suitable for routine molecular biology applications.
• Silica Membrane Technology: Utilizes silica membrane technology, eliminating the need for cumbersome steps associated with loose resins or slurries, streamlining the purification process.
• Immediate Usability: Purified plasmid DNA is immediately ready for downstream applications, saving time and effort.
• No Phenol Extraction or Ethanol Precipitation: Bypasses the need for phenol extraction and ethanol precipitation, simplifying the workflow and reducing hands-on time.
• High-Quality DNA: Yields high-quality plasmid DNA eluted in a small volume of Tris buffer or water, ensuring optimal performance in downstream applications.

Specifications

Principle

• Alkaline Lysis: Begins with the alkaline lysis of bacterial cells, which breaks down the cell membrane and releases plasmid DNA into the lysate.
• Silica-Based DNA Binding: The released DNA is adsorbed onto the silica membrane in the presence of high salt, facilitating efficient binding of DNA to the membrane surface.
• Three Basic Steps: The procedure involves three main steps: (1) Preparation and clearing of the bacterial lysate using an alkaline method, (2) Transfer of the cleared lysate to a column for DNA binding, and (3) Washing to remove proteins and other impurities.
• Silica Membrane: Utilizes a unique silica membrane in the kit, which completely replaces traditional glass or silica slurries typically used in plasmid DNA minipreps.
• Elution with Low-Salt Buffer: After washing to remove impurities, nucleic acid, including plasmid DNA, is eluted from the silica membrane using a low-salt buffer containing 10mM Tris, pH 9.0, and 0.5mM EDTA.

Advantages

• High purity – purified plasmid can be directly used in sequencing, enzyme digestion PCR, etc.
• Fast – it takes only 1 minute to obtain supernatant by optimized solution (10 minutes for other brands)
• High yield – up to 35µg plasmid can be bound in one column

Kit Contents

Storage and Stability

• Storage Conditions: Kit components can be stored dry at room temperature (15-25°C) and remain stable for a minimum of 18 months under these conditions.
• Buffer Precipitates: In case of any precipitates forming in the buffers, warm them at 37°C to dissolve effectively.
• Buffer P1 Stability: After the addition of RNase A, Buffer P1 remains stable for up to 6 months when stored at 2-8°C.

Experiment Data

Purchase Guide