Product Overview
- Offers fast and easy purification methods for total DNA.
- Ensures reliable PCR and Southern Blotting results.
- Capable of purifying various types of DNA, including genomic, viral, and mitochondrial.
- Can purify DNA from diverse sources: tissue, whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, and cultured cells.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from blood, tissue, culture cells, swab, and blood spots using 96 plate |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification method | 96 well plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Blood, serum, plasma, milk, saliva, and other liquid samples and cultured cells |
| Sample amount | |
| Elution volume | |
| Time per run | |
| Liquid carrying volume per column | |
| Binding yield of column |
Principle
- Utilizes silica column purification method.
- The sample is lysed and digested with lysate and protease, releasing DNA into the lysate.
- Lysate is transferred to an adsorption column.
- Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed through filtration.
- Proteins and other impurities are washed away.
- Nucleic acid is finally eluted with low-salt buffer (10mm Tris, pH 9.0, 0.5mm EDTA).
Advantages
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
- High throughput – 96 samples can be processed simultaneously
Kit Contents
| Contents | D311701 | D311702 |
| Purification Times | 1 x 96 | 4 x 96 |
| HiPure gDNA Plate | 1 | 4 |
| 96 well Plate (2.2ml) | 1 | 4 |
| 1.6ml Collection Plate | 1 | 4 |
| 0.5ml Collection Plate | 1 | 4 |
| Silicon Seal Tape | 1 | 4 |
| Seal Film | 5 | 25 |
| Buffer ATL | 30 ml | 100 ml |
| Buffer AL | 30 ml | 100 ml |
| Buffer DW1 | 60 ml | 250 ml |
| Buffer GW2 | 50 ml | 2 x 100 ml |
| Proteinase K | 50 ml | 200 ml |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Buffer AE | 30 ml | 120 ml |
Storage and Stability
- Proteinase K should be stored at 2-8°C upon arrival.
- Short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect its performance.
- The remaining kit components can be stored at room temperature (15-25°C) and remain stable for at least 18 months under these conditions.
Purchase Guide
| Name | CAT NO | Sample amount | Leukocyte protocol* | Colum type | Elution volume | Average yield | Time per run |
| HiPure Blood DNA Mini Kit | D3111 | 10-200μl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| HiPure Tissue&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure Tissue&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure Tissue&Blood DNA 96 Kit | D3117 | 1-200μl | 1ml | 96 well plate | 3-8μg/200μl |
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