- Challenges in DNA Extraction from Blood Samples:
- High Infectious Risk: Blood samples pose a significant infectious hazard to operators and the environment.
- Complex DNA Source: Blood DNA contains various types such as mitochondrial, genomic, and circulating DNA, leading to potential loss during extraction.
- Presence of Impurities: Blood samples contain impurities and inhibitory factors that can interfere with DNA extraction and downstream applications.
- Existing Extraction Methods:
- Phenol-chloroform extraction, salting out, etc.
- These methods typically involve pre-treatment steps to remove red blood cells and isolate white blood cells, which may result in loss of nucleic acid information.
- Product Benefits:
- Fast and Easy Purification: Provides a rapid and straightforward method for total DNA purification.
- Suitability: Purifies total DNA (genomic, viral, mitochondrial) from whole blood, tissue, and cultured cells.
- Application: Ensures reliable DNA for PCR and Southern blotting assays.
Specifications
| Features | Specifications |
| Main Functions | Isolation of total DNA from 10ml blood and 1g tissue using Maxi column |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification method | Maxi spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Tissue, cells, blood, saliva, swabs, blood spots, semen, and other clinical samples |
| Sample amount | 3-10ml |
| Elution volume | ≥700μl |
| Time per run | ≤90 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 5mg |
Principle
- Cell Breakdown: Your sample is lysed (broken open) and digested with enzymes to release DNA into solution.
- Capture: The mixture is passed through a silica column. DNA sticks to the silica membrane, while proteins and other impurities don’t.
- Washing Away Impurities: The column is washed to remove any remaining proteins and other unwanted molecules.
- DNA Release: Pure DNA is eluted (washed off) the silica membrane with a special buffer solution. This purified DNA is then ready for further analysis.
Advantages
- High-quality DNA – meets a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
- Fast – without separation of leukocytes, organic extraction, or ethanol precipitation
- Simple – all nucleic acids can be obtained by direct digestion
- Pertinence – specially designed for isolating DNA from 3-10ml blood and related body fluids
- Wide applicability – handle a variety of liquid samples
- Economy – excellent cost-effectiveness performance
Kit Contents
| Contents10 | 10 preps | 50 perps |
| Purification Times | 10 | 50 |
| HiPure gDNA Maxi Columns | 10 | 50 |
| 50ml Collection Tubes | 20 | 100 |
| Buffer ATL | 120 ml | 550 ml |
| Buffer AL | 120 ml | 2 x 300 ml |
| Buffer GW1* | 53 ml | 220 ml |
| Buffer GW2* | 25 ml | 110 ml |
| RNase A | 40 mg | 180 ml |
| Proteinase K | 120 mg | 540 mg |
| Protease Dissolve Buffer | 12 ml | 50 ml |
| Buffer AE | 30 ml | 120 ml |
Storage and Stability
Storage Recommendations:
- Proteinase K and RNase A: Upon arrival, store at 2-8°C. Short-term storage (up to 12 weeks) at room temperature (15-25°C) is acceptable without affecting performance.
- Remaining Kit Components: Can be stored at room temperature (15-25°C) and remain stable for at least 18 months under these conditions.
Purchase Guide
| Name | CAT NO | Sample amount | Leukocyte protocol* | Colum type | Elution volume | Average yield | Time per run |
| HiPure Blood DNA Mini Kit | D3111 | 10-200μl | 1ml | 2ml column | ≥20μl | 5-9μg/200μl | ≤30 minutes |
| HiPure Tissue&Blood DNA Midi Kit | D3113 | 0.2-2ml | 10ml | 1.5ml column | ≥300μl | 20-40μg/1m | ≤80 minutes |
| HiPure Tissue&Blood DNA Maxi Kit | D3115 | 3 -10ml | 10ml | 15ml column | ≥700μl | 20-40μg/1m | ≤90 minutes |
| HiPure Tissue&Blood DNA 96 Kit | D3117 | 1-200μl | 1ml | 96 well plate | 3-8μg/200μl |
