Introduction
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, urine, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.
Details
Specifications
| Features | Specifications |
| Main Functions | Extract viral RNA/DNA from non-cell/low cell content biological samples |
| Applications | RT-PCR，PCR，NGS |
| Products | Viral total nucleic acid |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Cell-free body fluid such as plasma, serum, soaking solution and tissue homogenate supernatant |
| Sample amount | 200μl |
| Yield | 2-10μg |
| Elution volume | ≥30μl |
| Time per run | ≤30 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Principle
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA / RNA is released into the lysate. Transfer to an adsorption plate and filter column. DNA/RNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, DNA / RNA was finally eluted with low-salt buffer (10 Mm Tris, pH 8.0).
Advantages
- Fast – several samples can be extracted in 20 minutes by column method
- High quality – high purity total RNA can be directly used in various sensitive downstream applications
- Safe – no phenol chloroform extraction required
- Sensitive – DNA/RNA can be recovered at the level of PG
- High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid
Kit Contents
| Contents | IVD4173 |
| Purification Times | 100 Preps |
| HiPure Viral Mini Column | 100 |
| 2ml Collection Tubes | 200 |
| PK/Carrier RNA | 50 mg |
| Protease Dissolve Buffer | 5 ml |
| Buffer AL | 30 ml |
| Buffer MW1* | 44 ml |
| Buffer MW2* | 50 ml |
| RNase Free Water | 15 ml |
Storage and Stability
Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
Experiment Data
Reviews
There are no reviews yet.