Introduction
This product provides fast and easy methods for the purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, and cultured cells.
Relevant reagents were pre-packed in 96-well plates following the optimal protocol.
Specifications
Features Specifications
Main Functions Isolation total DNA from 200μl whole blood
Applications PCR, southern bolt and virus detection, etc
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Whole blood, concentrated blood, buffy coat, and other samples
Sample amount 200μl
Elution volume ≥70μl
Time per run ≤40 minutes
Principle
This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with a washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally, DNA was eluted by an Elution Buffer.
Advantages
- High binding force – suitable for handling DNA-rich samples, such as whole blood, buffy coat, concentrated blood, etc.
- Fast – polydisperse magnetic beads, fast magnetic response, and short extraction time
- High purity – the obtained DNA can be directly used for second-generation sequencing, PCR-based detection, gene bank, etc.
Kit Contents
| Contents | D631001 | D631002 | D631003 |
| Purification Times | 48 | 96 | 480 |
| MagPure Particles | 1.7 ml | 3.5 ml | 17 ml |
| Proteinase K | 24 mg | 50 mg | 220 mg |
| Protease Dissolve Buffer | 1.8 ml | 3 ml | 15 ml |
| Buffer MLA | 30 ml | 60 ml | 270 ml |
| Buffer DW1 | 60 ml | 120 ml | 550 ml |
| Buffer CW | 30 ml | 60 ml | 270 ml |
| Buffer BW3 | 30 ml | 60 ml | 270 ml |
| Elution Buffer | 15 ml | 30 ml | 100 ml |
Proteinase K, MagPureParticles should be stored at 2-8°C upon arrival. However, short-term storage(up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C)and are stable for at least 18 months under these conditions.
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