Introduction
The Kit is specially designed for simultaneous purification of genomic DNA and total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Purified DNA/RNA is suitable for use in applications such as real-time PCR and Pyrosequencing.
Details
Specifications
| Features | Specifications |
| Main FunctionsC | Co-isolation total RNA and DNA from FFPE tissue |
| Applications | RT-PCR, cDNA synthesis, PCR and second-generation sequencing, etc. |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor and pipetting workstation |
| Sample type | FFPE slice, FFPE puncture sample, embedded tissue |
| Sample amount | No more than six 10µm sections of 150 mm2 surface area or three 20µm sections of 150 mm2 surface area. |
Principle
FFPE samples are incubated in an optimized lysis buffer, which results in the release of RNA and precipitation of DNA. After centrifugation, the RNA-containing supernatant and DNA-containing pellet are then processed separately to purify RNA and DNA. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by RNase Free Water.
Advantages
- Post digestion sorting – higher DNA and RNA yields
- Economy – the price is much lower than imported reagents
Kit Contents
| Contents | IVD3026 |
| Purification Times | 200 Preps |
| MagBind Particles | 9.0 ml |
| Proteinase K | 180 mg |
| Protease Dissolve Buffer | 10 ml |
| Buffer DPS | 150 ml |
| Buffer FRL | 40 ml |
| Buffer ATL | 40 ml |
| Buffer AL | 80 ml |
| Buffer BXW1* | 110 ml |
| RNase Free Water | 30 ml |
Storage and Stability
Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
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