Introduction
This product is suitable for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen, and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA, and so on.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation of total DNA from blood, buffy coat, tissue, and other samples |
| Applications | Second-generation sequencing, PCR, real-time PCR, etc. |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Anticoagulant blood, concentrated blood, buffy coat, lymphocytes and cultured cells |
| Sample amount | Whole blood :< 200μl; Saliva / swab:< 400μl; Tissue :< 20mg |
| Yield | 0.1 – 50μg |
| Elution volume | |
| Time per run |
Principle
This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of lysate and protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with a washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally, DNA was eluted with an Elution Buffer.
Advantages
- High purity – OD 260 / 280: 1.7- 1.9, OD 260 / 230: 1.5 – 2.0
- Economy – less than 50% of the price of Qiagen and other imported products
- High yield – most optimal process, ensuring the recovery of up to 90%
- Strong processing ability – samples including animal blood, cultured cells, animal tissues, etc.
Kit Contents
| Contents | IVD3102 |
| Purification Times | 200 |
| MagPure Particles | 5 ml |
| Proteinase K | 100 mg |
| Protease Dissolve Buffer | 10 ml |
| Rnase A | 40 mg |
| Buffer ATL | 60 ml |
| Buffer AL | 60 ml |
| Buffer BD* | 20 ml |
| Buffer BW1* | 110 ml |
| Elution Buffer | 30 ml |
Storage and Stability
Proteinase K, RNase A, MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
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