Notice: You need an Immunofluorescence Analyzer to run the tests with this kit
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SPECIFICATION
10 Test/Box
Cat.No.:MG-CA-R033
INTENDED USE
The Feline FPV/FHV/FCV Ab test is a quantitative fluorescence immunoassay used with an Immunofluorescence Analyzer. It determines the concentration of antibodies to feline panleukopenia virus (FPV), feline herpesvirus (FHV), and feline calicivirus (FCV) in serum or plasma samples.
For in vitro diagnostic use only. For professional use only.
TEST PRINCIPLE
- This test employs a quantitative double antigen sandwich fluorescence immunoassay technique.
- The fluorescent signal intensity reflects the amount of FPV-Ab, FHV-Ab, FCV-Ab captured. The concentration is expressed as Tu/ml.
MATERIAL
| Material Provided | Material Required But Not Provided |
|---|---|
| 10 individual sealed pouches, each containing: ■ Test Device of FPV-Ab, FHV-Ab, and FCV-Ab (antibody test for Feline Panleukopenia Virus, Feline Herpesvirus, and Feline Calicivirus) ■ Desiccant pouch | 1. Immunofluorescence Analyzer (for reading test results) |
| One ID Chip | 2. Timer |
| Instructions for Use | 3. Pipette (already provided) |
| 10 tubes of FPV/FHV/FCV Ab Sample Buffer | 4. Centrifuge (not typically required for antibody tests) |
| 10 Pipette tips |
STORAGE AND STABILITY
- Store the test kit at 4～30℃ up to the expiration date.
- The test should operate at 18 ~ 28℃ after the Test Device opening.
- Once the pouch is opened, the test should be performed within 30 minutes.
SPECIMEN COLLECTION AND PREPARATION
The test can be performed with serum or plasma.
- Separate serum or plasma (EDTA anticoagulant is recommended) from blood within 3 hours after blood collection. If a specimen appears to be severely hemolyzed, another specimen should be obtained and tested.
- Optimally, the test should be performed immediately after the specimen collection. If the test cannot be performed within 3 hours after blood collection, store the specimen at 2～8℃ for no longer than 72 hours. For long-term storage, specimens shall be kept below -20℃.
Bring all materials to room temperature before use. Frozen specimens must be completely thawed and mixed well prior to testing. Specimens should not be frozen and thawed repeatedly. Only clear, non-hemolyzed specimens can be used.
TEST PROCEDURE
Preparation:
- Set a Test Device on a clean, level horizontal table.
- Insert the ID Chip into the meter and click “Read ID chip.” Make sure the Test Device lot number matches the ID Chip number.
Sample Preparation:
- Pipette 20 μl of prepared sample into FPV/FHV/FCV Ab Sample Buffer.
- Gently mix well. Avoid vigorous agitation and foaming.
Run the Test:
- Choose between Standard Test (a) or Quick Test (b).
(a) Standard Test: * Pipette 100 μl of mixed sample into the sample well (S) of the FPV-Ab Test Device. * Click “Standard Test,” insert the FPV-Ab Test Device into the meter holder, and click “Start Test.” Select sample type “Serum/Plasma.” The meter will countdown and read the result automatically. * Repeat for FHV-Ab and FCV-Ab Test Devices, clicking “Start Test” for each after inserting them into the holder.
(b) Quick Test: * Pipette 100 μl of mixed sample into the sample well (S) of the FPV-Ab Test Device. * Click “Quick Test,” start the timer immediately. * Leave the FPV-Ab Test Device at room temperature (18°C – 28°C) for 10 minutes. * Insert the FPV-Ab Test Device into the meter holder and click “Start Test.” Select sample type “Serum/Plasma.” The instrument will scan the Test Device automatically and show the test result. * Repeat for FHV-Ab and FCV-Ab Test Devices, inserting them in turn and clicking “Start Test” for each.
- Results:
- Results are displayed on the main screen. Printing is automatic or can be done by clicking “Print.”
QUALITY CONTROL
INTERPRETATION
Reference range of FPV/FHV/FCV in feline serum or plasma.
- Detection range :
① FPV-Ab：4.5 ~640 Tu/ml
② FHV-Ab：5.0 ~640 Tu/ml
③ FCV-Ab：5.0 ~640 Tu/ml
- Reference range:
| Result(Tu/ml) | Level | Suggestion | ||
| FPV-Ab | FHV-Ab | FCV-Ab | ||
| ≤9 | ≤10 | ≤10 | 0 | No antibody protection vaccination is recommended |
| 9～50 | 10～15 | 10～30 | 1 | The antibody protection is weak, so it is recommended to carry out supplementary immunization or adjust the immunization program |
| 50~ 150 | 15～45 | 30～90 | 2 | |
| 150~ 300 | 45~ 100 | 90~ 200 | 3 | Moderate antibody protection, strengthen antibody level monitoring once every 6 months or so |
| 300~ 500 | 100~ 150 | 200~ 300 | 4 | |
| >500 | >150 | >300 | 5 | The antibody protection is strong, and the antibody level is tested every year to ensure the vaccine protection in time |
Note
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Each laboratory should establish a reference range that represents the population to be evaluated.
WARNINGS AND LIMITATIONS OF THE PROCEDURE
- This kit is for in vitro diagnostic use only.
- Inspect the packaging and labels before use. Do not use it if the pouch is broken, torn, or not well sealed, or if the vial looks damaged or leaked.
- Do not use the test device beyond the expiration date.
- One pipette tip should be used for one specimen only.
- There is the possibility that factors such as technical or procedural errors and additional substances in blood specimens that are not listed in the text may interfere with the test and cause erroneous results.
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