- Sample Handling:
- A. Material Collection & Storage:
- If collected fresh materials cannot be used immediately, cool them in liquid nitrogen and store them at -80°C. Dried materials can be stored in the refrigerator.
- B. Collection Preference:
- Collect fresh material whenever possible, as it contains fewer polysaccharides and polyphenols.
- C. Fungal Sample Collection:
- Fungi can be collected by centrifugation from liquid media.
- A. Material Collection & Storage:
- Sample Preparation:
- Grind about 100 mg of fresh samples or no more than 20 mg of dry matter with liquid nitrogen.
- Add 400 μl Buffer I and 10 μl RNaseA (10 mg/ml) to ensure no lumpy tissue in the sample, which can reduce DNA yield.
- Incubate at 65°C for 2-10 minutes, invert, and mix 2-3 times during this period. Extend incubation time if the sample is difficult to lyse, but not more than 1 hour.
- Add 130 μl Buffer II, mix well, and incubate on ice for 5 minutes to precipitate polysaccharides and proteins.
- Purification:
- Add the lysate to the purification column and centrifuge at 13,000 rpm for 2 minutes.
- Transfer to Reagent Plate:
- Add the centrifuged supernatant from the previous step to the 96-well plate.
- Follow specific steps outlined in the automatic extraction protocol for further processing.
Pre-processing of plant sample genomic DNA extraction 96 Samples
$225.00
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Description
Additional information
| Weight | 0.7 kg |
|---|---|
| brand name |
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