Introduction
- RNA Clean Beads:
- Utilize paramagnetic bead technology for high-throughput purification of RNA or cDNA.
- Designed for in vitro applications such as transcription, antisense RNA (aRNA) amplification, and RNA and cDNA probe synthesis.
- Applications:
- The resulting purified product can be used in various applications, including:
- PCR and RT-PCR
- Probes for microarray or macroarray
- RNase protection assays
- Transfection for RNAi experiments
- cDNA synthesis and labeling
- The resulting purified product can be used in various applications, including:
Details
Specifications
| Features | Specifications |
| Main Functions | Recovery of RNA from RNA reaction solution by magnetic bead method (Replace Beckmen or agencourt AmPure XP) |
| Applications | Advanced applications, such as sequencing, genechip, fluorescence quantification, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | RNA products, restriction endonuclease systems, or other enzymatic reaction solutions |
| Sample amount | 20-100µl |
| Recovery | 90% |
| Elution volume | ≥20μl |
| Operation time | ≤50 minutes |
Principle
RNA Clean Process:
- Selective Binding:
- Utilizes an optimized buffer to selectively bind RNA or cDNA to paramagnetic beads.
- Contaminant Removal:
- Excess oligonucleotides, nucleotides, salts, and enzymes are removed through a simple washing procedure.
Advantages
- High recovery – recovery of RNA up to 90%
- High throughput – using magnetic beads purification technology
- Simple – simplified operation process, combination – washing – elution
Kit Contents
| Contents | BRP-5 | BRP-50 | BRP-500 |
| RNA Clean Beads | 5 ml | 50 ml | 500 ml |
Storage and Stability
DO NOT FREEZE.
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