RNase A

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Introduction

Characteristics of RNase A:

1. Enzyme Type:
   • RNase A is an endoribonuclease that specifically degrades single-stranded RNA.
2. Specificity:
   • It specifically cleaves RNA at cytosine (C) and uracil (U) residues.
3. Action Mechanism:
   • The enzyme cleaves the phosphodiester bonds between the 5′-ribose of a nucleotide and the phosphate group attached to the 3′-ribose of an adjacent pyrimidine nucleotide.
   • This action initially forms a 2′,3′-cyclic phosphate, which is subsequently hydrolyzed to a 3′-nucleoside phosphate.
4. Structure:
   • RNase A is a single-chain polypeptide that includes 4 disulfide bridges, contributing to its stability.
5. Optimal Activity:
   • The highest enzymatic activity of RNase A is observed when acting on single-stranded RNA.

Applications of RNase A:

Primary Use:

• • RNase A is extensively used to remove RNA from preparations of plasmid DNA, ensuring that the DNA samples are free of RNA contamination.

Activity Across Different Conditions:

Salt Concentration Influence:

• • At low salt concentrations (0 to 100 mM NaCl), RNase A can cleave both single-stranded and double-stranded RNA, and the RNA strand in RNA-DNA hybrids.
  • At higher salt concentrations (0.3 M NaCl or more), its activity becomes specific to single-stranded RNA.

Details

Specifications

Advantages

• High activity, >50 U/mg
• High cost performance
• High purity, low impurity protein pollution
• No residual Dnase or trace Dnase

Ordering information

Storage and Stability

Magen recommends storage at -20-8 °C. When stored at 2-8 °C, the product retains activity for at least 2 years.