Sanshibio DNA Marker (1kb)

DNA Marker (1kb)

Item No：DA009       Specifiation：250μL        Storage：-20℃，Store at 4°C after opening or melting

Product introduction：

1kb DNA Marker is a DNA solution containing 1× Loading Buffer, which allows for direct electrophoresis with great convenience, using 5μL. 1kb DNA Marker consists of fourteen DNA fragments with sizes of 10000bp, 8000bp, 6000bp, 5000bp, 4000bp, 3500bp, 3000bp, 2500bp, 2000bp, 1500bp, 1000bp, 750bp, 500bp, and 250bp, totaling 14 bands. When 5μL is used for electrophoresis, the DNA content of the 6000bp, 3000bp, and 1000bp fragments is approximately 60ng, and the rest of the bands are approximately 30ng.

Product contents：

Storage：

-20℃ for long-term storage. After opening or melting, store at 4℃ for 1 year. Please use as soon as possible after opening to avoid contamination and repeated freezing and thawing.

Usage precautions:

• When the width of the sample wells in electrophoresis is less than 6mm, clear bands can be obtained by loading 5μL of the product. If the sample wells are widened, it is necessary to appropriately increase the amount of Marker product used for loading.
• For DNA electrophoresis, the purity of Agarose significantly affects the clarity of DNA bands. Therefore, it is advisable to use high-quality Agarose with a recommended gel concentration of 1% – 2%.
• The concentration of Agarose used in the electrophoresis process is closely related to the separation performance of DNA fragments. Higher Agarose concentrations are more suitable for separating short DNA fragments, while lower Agarose concentrations are more favorable for the separation of long DNA fragments.

Agarose Electrophoresis Map

1%Agarose gel electrophoresis image