Sanshibio GS 15000 DNA ladder

GS 15000 DNA ladder

Item No：DA018            Specifiation：250μL               Storage：-20℃

Product introduction：

GS 15000 DNA ladder is a DNA solution pre-loaded with 1× Loading Buffer, allowing for convenient direct electrophoresis with a 5μL sample volume. Comprising 7 linear double-stranded DNA fragments, each band of the ladder contains approximately 30ng when 5μL is loaded, with the bright bands reaching around 80ng. Following electrophoresis on GelRed and GelGreen gels, the DNA bands exhibit sharp clarity, excellent gel lane background, high and uniform brightness, and the absence of anomalies such as trailing or smearing.

Product contents：

Storage：

Store at -20℃ for long-term preservation. After opening, please use as soon as possible to avoid contamination and repeated freeze-thaw cycles.

Usage precautions:

1. When the well width for electrophoresis is less than 6mm, clear bands can be obtained by loading 5μL of the product for each run. If the well width increases, it is necessary to appropriately increase the amount of Marker product.
2. For DNA electrophoresis, the purity of Agarose has a significant impact on the clarity of DNA bands. Therefore, it is recommended to choose high-quality Agarose with a gel concentration of 0.6%-1%, and there is a relatively lower requirement for gel concentration. When the gel concentration is too high, large DNA fragments may not separate well, and it is not suitable for use with TBE electrophoresis buffer.
3. The GS series DNA ladder is compatible with high-molecular-weight nucleic acid stains, such as GelRed (including various GelRed modified nucleic acid stains) and GelGreen gel staining. For small-molecule nucleic acid stains like EB, Goldview, using the GS series DNA ladder may result in migration rate differences, so it is advisable to choose our company’s conventional DNA Marker series.

Agarose Electrophoresis Map

0.7%Agarose gel electrophoresis image