Solar Protein Precast-Gel (Native) 8-20%, 15 wells

A Protein Precast-Gel (Native) 8-20%, 15 wells is a pre-made polyacrylamide gel designed for the separation of native proteins based on their size without denaturation. These gels are commonly used in techniques like native polyacrylamide gel electrophoresis (PAGE) or blue native PAGE.

Features of Protein Precast-Gel (Native) 8-20%, 15 wells:

1. Gradient Composition: The gel has a gradient composition of acrylamide, typically ranging from 8% to 20%, which allows the separation of proteins over a broad size range.
2. 15 Wells: The gel is precast with 15 wells, providing the user the ability to load and separate multiple protein samples simultaneously.
3. Native Conditions: The gel is designed to maintain native conditions, preserving the native structure and activity of proteins.

How to Use Protein Precast-Gel (Native) 8-20%, 15 wells:

1. Gel Preparation:
   • Remove the Protein Precast-Gel from its packaging. Place the gel into the gel-running apparatus or electrophoresis tank, ensuring proper alignment with the electrodes.
2. Sample Loading:
   • Prepare your protein samples by mixing them with a suitable loading buffer that is compatible with native electrophoresis. Load the protein samples into the wells of the gel using a micropipette.
3. Electrophoresis:
   • Submerge the gel in the electrophoresis buffer in the electrophoresis tank. Apply an electric current according to the manufacturer’s recommendations. Native electrophoresis typically runs at a lower voltage compared to denaturing gels.
4. Run Duration:
   • Run the electrophoresis until the proteins have migrated sufficiently through the gel based on the desired resolution. The run time will depend on the specific apparatus and conditions used.
5. Gel Staining:
   • After electrophoresis, the gel can be stained to visualize the separated proteins. Common stains for native gels include Coomassie Brilliant Blue or silver staining.
6. Destaining and Imaging:
   • Destain the gel if necessary, and then image the gel using a gel documentation system or other appropriate imaging methods.
7. Analysis:
   • Analyze the gel to determine the molecular weights and relative quantities of the separated proteins. Compare the results with protein standards run on the same gel.