Serum Ferri Ion Content Assay Kit
Note: Take two or three different samples for prediction before test.
Operation Equipment: Spectrophotometer
Cat Number: BC1730
Size: 50T/48S
Components:
Reagent I: Powder×2, storage at 4℃. Add 10 mL distilled water before use.
Reagent Ⅱ: Powder×2, storage at 4℃. Add 313 μL glacial acetic acid and 10 mL distilled water before use. Standard Solution: Liquid 3 mL×1, 1000 μmol/L Fe3+ standard solution, storage at 4℃. Add distilled water dilute 8 times to form a standard solution of 125 μmol/L before use.
Product Description:
Serum iron is the iron bound with transferrin in blood, which is often used to distinguish non-iron deficiency anemia and iron-deficiency anemia.
Fe3+ is reduced by sodium sulfite to Fe2+, which reacts with 2,2-dipyridine-bipyridine, have an absorption peak at 520 nm. According measure absorbance at 520 nm can reflect serum iron concentration.
Reagents and Equipment Required but Not Provided.
Spectrophotometer, centrifuge, glacial acetic acid, adjusted transferpettor, 1 mL glass cuvette, chloroform and distilled water.
Procedure:
- Preheat the spectrophotometer for 30 min, adjust wavelength to 520 nm, set zero with distilled
- Dilute standard solution to 125 μmol/L with distilled
- Add reagents with the following list:
| Reagent Name (μL) | Blank tube (AB) | Test tube (AT) | Standard tube (AS) |
| Distilled water | 400 | ||
| Standard solution (125 μmol/L) | 400 | ||
| Serum(plasma) | 400 | ||
| Reagent I | 400 | 400 | 400 |
| Reagent II | 400 | 400 | 400 |
| Mix thoroughly, incubate in boiling water bath for 5 min, cooling liquid. Add 200 μL chloroform (required but not provided). Mix thoroughly, room temperature, 10000 rpm centrifuge for 10 min. Take 700 μL supernatant to 1 mL glass cuvette. Measure absorbance at 520 nm. Recorded as AB, AT, AS. | |||
Calculations
Serum iron (μmol/L)=[Cs×(AT-AB)÷(As-AB)]= 125×(AT-AB)÷(As-AB)
Cs: Fe3+ standard solution, 125 μmol/L.
Note:
- There is less iron in the serum, so the vessels (EP tubes) should be noted to avoid iron contamination.
- Reagent I and Reagent II are unstable. It needs to be prepared when the solution will be used, and the newly prepared reagent can only be used on the same day.
- When At- Ac＞5, please dilute the Serum to appropriate concentration with distilled water.
Technical Specifications：
Minimum Detection Limit：3.212 μmol/mL
Linear Range：3.9-250 μmol/mL
Related products：
BC2860/BC2865 Serum Total Iron Binding Capacity(TIBC) Assay Kit
BC0720/BC0725 Blood Calcium Content Assay Kit
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