HiPure Циркулирующая ДНК Midi Spin Kit D (Протокол спина)

Введение

HiPure Circulating DNA Midi Kit, designed to efficiently purify short fragments of cell-free DNA (вкДНК) from human biofluids for clinical use:

• Цели:
  • Tumor-derived cfDNA fragments and mRNAs in blood
  • Fetal nucleic acids in maternal blood
• Типы образцов:
  • Human plasma
  • Human serum
  • Human urine (fresh or frozen once)
• Focus on Short DNA: Captures short nucleic acids (<1000б.п.) commonly found in biofluids.
• Efficient Purification: Effectively recovers cfDNA from various biofluids.
• Clinical Applications: Purified cfDNA is suitable for approved in vitro diagnostic (ИВД) tests.

Преимущества:

• Optimized for Short cfDNA: Ideal for capturing short DNA fragments relevant for cancer biomarkers and liquid biopsies.
• Широкая совместимость образцов: Works with various human biofluids, offering flexibility in sample selection.
• Larger Scale Purification: The Midi Kit format allows the processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
• Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.

This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (НИПТ), and liquid biopsies, ultimately aiding in patient diagnosis and management.

Подробности

Технические характеристики

Принцип

This product utilizes silica gel columns for efficient DNA extraction:

1. Lysis & Digestion: The sample undergoes treatment with a special solution and enzyme to break open cells and degrade proteins, releasing DNA.
2. Binding to Silica: The mixture is transferred to a column. The silica membrane in the column captures DNA molecules, while other substances pass through.
3. Мойка: Wash buffers to remove leftover proteins and cellular debris.
4. Элюирование: A low-salt buffer elutes the purified DNA from the silica, collecting it in a separate tube.

This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.

Преимущества

• Высокая доходность – самый оптимальный процесс, свободная ДНК (>50б.п.) можно получить в максимальной степени
• Высокая концентрация – низкий объем элюирования, обеспечение высокой концентрации нуклеиновой кислоты
• Высокая чистота – метод связывания с низким содержанием спирта, полное удаление ингибиторов и белковых загрязнений
• Высокое восстановление – DNA can be recovered at the level of PG by silica gel column purification

Содержимое комплекта

Хранение и стабильность

• Протеиназа К & РНК-носитель: Fridge (2-8°С) for best results.
• Все остальное: Комнатная температура (15-25°С) is fine.
• Важный: If storing cold, warm buffers before use.