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Он прибудет в течение 7-10 дни. Стоимость доставки включена в стоимость товара.
Введение
РНКаза А is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5′-ribose of a nucleotide and the phosphategroup attached to the 3′-рибоза соседнего пиримидинового нуклеотида. The resulting 2′,3′-cyclic phosphate is hydrolyzed to the corresponding 3′-нуклеозидфосфат. The highest activity is exhibited with single-stranded RNA. RNase A is a single chain polypeptide containing 4 дисульфидные мостики.
A major application for RNase A is the removal of RNA from preparations of плазмидная ДНК. The enzyme is active under a wide range of reaction conditions. При низкой концентрации соли (0 к 100 мМ NaCl), RNase A cleaves single-stranded and double-stranded RNA as well as the RNA strand in RNA-DNA hybrids. Однако, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.
Подробности
Технические характеристики
| Функции | Технические характеристики |
| Чистота | ≥60% RNase A basis (Паспорт безопасности-СТРАНИЦА) |
| Enzymatic activity | >50 Единицы Кунитца/мг белка |
| Optimum reaction temperature | 60°С (effective active temperature is 15-70°C) |
| Условия перевозки | Normal temperature transportation |
| Условия хранения | -20-8°С, сухое хранение, длительное хранение должно осуществляться при температуре -20°C.. |
| Приложение 1: adding in the extraction process | 1. Plasmid Extraction: add RNase A (25мг/мл) to buffer P1 with a final concentration of 100-300μg/ml. 2. экстракция ДНК: add RNase A (25мг/мл) to the digestion solution with a final concentration is 100-400μg/ml, mix well and place at room temperature for 10-15 минуты. when SDS/СТАБ in lysate exceeds 2%, RNase activity will be significantly inhibited; Гуанидин salt(>4M guanidine hydrochloride or >3М гуанидинизотиоцианат) also significantly inhibited RNase A. When RNase A is added to the lysate, the RNase digestion effect can be extracted by appropriately diluting to reduce the concentration of SDS, CTAB and guanidine salt. |
| Приложение 2: | 1. Remove RNA contamination from crude genomic DNA products: add DNase free RNase A (10мг/мл) to crude DNA products with a final concentration of 10-100μg/ml. After mixing, place at room temperature for 10 минуты. 2. Remove RNA contamination from plasmid DNA products: add DNase free RNase A (10мг/мл) to crude DNA products with a final concentration of 10μg/ml. After mixing, it can be directly used for sequencing at room temperature for 10 минуты. |
Информация для заказа
| Содержание | C12123 | C12124 | C12128 | C12129 |
| RNase A Solution (25мг/мл) | 10 мл | 100 мл | ||
| DNase Free RNase A Solution (10мг/мл) | 10 мл | 100 мл |
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