Набор для выделения тотальной ДНК и РНК животных Sanshibio, 50 т.

Total DNA/Набор для экстракции РНК（Column Extraction Method）

предмет номер：DP201 Спецификация：50Т Хранилище：РТ

Principle of the Product:

The Total DNA/RNA Extraction Kit is based on the silica column purification method. Under the action of lysis buffer, samples are lysed, releasing nucleic acids into the lysate. Nucleic acids are adsorbed onto the membrane of the silica column while passing through it in a high-salt environment, while proteins are not adsorbed and are removed. The nucleic acid-bound membrane is washed to remove residual proteins and salts, и наконец, the nucleic acids adsorbed on the membrane are eluted using DEPC-treated water. The resulting nucleic acids are of high purity and can be directly used for various downstream experiments.

Описание продукта:

The Total DNA/RNA Extraction Kit is suitable for rapidly extracting high-purity total nucleic acids, including viruses, бактерии, parasites, and other biological entities, from samples such as tissues, кровь, secretions, and excreta. The kit utilizes silica column purification technology, eliminating the need for toxic phenol-chloroform extraction and time-consuming alcohol precipitation during the extraction process. The obtained nucleic acids can be directly used in a series of downstream experiments, включая ПЦР/ОТ-ПЦР, Southern hybridization/Northern hybridization, as well as LAMP/RT-LAMP assays.

Содержание продукта：

Примечание: Add anhydrous ethanol to the wash solution before initial use (добавлять 20 mL for 25T, добавлять 40 mL for 50T), and store at room temperature.

Хранилище：

This kit should be stored in dry conditions at room temperature (15-25°С) and can be preserved for 12 месяцы. Для длительного хранения, it should be kept at 2-8°C.

Operating steps:

• Базовые приготовления (Pre-processing):

Blood Samples: Брать >500µL of EDTA anticoagulated blood, центрифуга для 30 секунды, and collect 200µL of the supernatant for the following steps.

Tissue Samples: Take 0.05-1g of tissue sample, добавлять 5-10 times volume of physiological saline, grind to a homogenized mixture, and collect 200µL of the homogenate for the following steps.

Secretions, Stool Samples: Immerse a cotton swab in physiological saline, rotate and smear it at the sampling site. After collection, break off the swab head into a centrifuge tube containing 1mL of physiological saline, vortex for 30 секунды, and collect 200µL of the mixed solution for the following steps.

• Transfer 200µL of the prepared sample from step 1 to a 1.5mL centrifuge tube, add 500µL of lysis buffer, mix by inversion, let it stand for 5 minutes to lyse, and centrifuge at 10000rpm for 3 минуты.
• Transfer 400µL of the supernatant to a new 1.5mL centrifuge tube, add 200µL of anhydrous ethanol, vortex for 20 секунды.
• Place Tube A inside Tube B, transfer the entire mixture from step 3 into Tube A, and centrifuge at 10000rpm for 1 минута.
• Discard the filtrate from Tube B, place Tube A back into Tube B, add 600µL of wash solution to Tube A, and centrifuge at 10000rpm for 1 минута.
• Discard the filtrate from Tube B, place Tube A back into Tube B, and centrifuge at 10000rpm for 2 минуты.
• Place Tube A into a new 1.5mL centrifuge tube. Add 50µL of elution buffer to Tube A, let it stand for 1-2 минуты, centrifuge at 10000rpm for 1 минута. The resulting solution is the total nucleic acid solution of the sample. If not used immediately, store at -80°C.

Важная заметка (Please read this before using this reagent kit):

1. To ensure accurate test results, please strictly follow the instructions.
2. Tubes A and B inside the kit are disposable items, please do not reuse them.
3. All waste materials used for testing should be placed in a waste container containing disinfectant, soaked for disinfection. After the experiment, immediately disinfect the workbench with 1% sodium hypochlorite or 75% алкоголь.
4. During the experiment, personnel should wear gloves, masks, and lab coats, and avoid contact between reagents, образцы, and the body. It is recommended to minimize contact with the openings of Tube A and centrifuge tubes. If the sample solution adheres to the gloves or splashes during steps 1-4, gloves should be immediately changed and the splashed area cleaned. All materials in contact with pathogens should be disposed of properly.